Figure 4.4 Roles of the rate of flow of
substrate, rdif, and the rate of enzyme
reaction, rkin, on the observed reaction
rate, robs, as function of the substrate
modulus and α 2 , for an inhibition
Michaelis-Menten kinetics where α 0 =1
and α 1 =1. The heavy solid line refers
to rkin/rmax; the light solid lines refers
to robs/rmax and the broken lines refer to
rdif/rmax.
substrate concentration, the observed rate is higher than the expected rate of the
biocatalytic reaction, rkin. This happens since the surface substrate concentration is lower
than the bulk substrate concentration, thus resulting in a smaller inhibition effect.
The enzymatic isomerization of glucose into fructose is probably the most successful
industrial application of immobilised enzyme technology (Olsen, 1995). This reaction can
be described by a reversible form of Michaelis-Menten kinetics (Lee et al., 1976) which
does not fit the general equation (9). Considering the reversible reaction S↔P, the rate
expression will be
(11)where Seq is the substrate concentration at equilibrium, given by
Multiphase bioreactor design 100