Multiphase Bioreactor Design

Table 6.7 Optical resolutions, enantioselective

conversions and synthesis of peptides

Enzyme-reaction description Reference

Resolution of a racemic mixture of N-benzoyl tyrosine ethyl ester by
-chymotrypsin in a 2-phase MR

López et al., 1991; Matson

& López, 1990

Synthesis of (R) glycidol by the lipase catalysed resolution of
racemic glycidyl butyrate

López et al., 1990

Resolution of a racemic mixture of N-acetylt tyrosine ethyl ester by
a-chymotrypsin in a 2-phase MR

Matson & Quinn, 1986

Resolution of (±)-trans-3-(4-methoxyphenyl) glycidic acid methyl
ester by lipase from Serratia marcescens in a 2-phase MR

Matsumase et al., 1994,

Furui et al., 1996

Resolution of (±)-methoxyphenylglycidate methyl ester by lipase in
a 2-phase MR

Tosa & Shibatani, 1995

Resolution of racemic glycidyl butyrate by lipase in a 2-phase MR Wu et al., 1990, 1993

Resolution of racemic cyanomethyl [2-(4-isobutylphenyl)
propionate] by lipase in a hollow fibre reactor

Giorno et al., 1995

Resolution of 1-phenyl-l, 2 ethanediol by glycerol dehydrogenase
with NADH regeneration by lactate dehydrogenase

Liese et al., 1996

Resolution of (S)-(+)-2-(6-methoxy-2-naphtyl) propionic acid by
lipase immobilised on solid particles in a 2-phase MR

Xin et al., 2000

Enantioselective oxidation of methyl phenyl sulfid by
chloroperoxidase in a UF cell

Pasta et al., 1999

Peptide synthesis catalysed by a-chymotrypsin in reversed micelles Lüthi & Luisi, 1984

Production of -glutamyl-cysteine by -glutamyl-cysteine synthetase
with ATP (PEG bound) regeneration by acetate kinase

Berke et al., 1988

Large scale synthesis of kyotorphin (Tyr-Arg) catalysed by a-
chymotrypsin in a flat MR

Herrmann et al., 1991

Dipeptide (Phe-Leu) synthesis catalysed by a-chymotrypsin in
TTAB/octanol/hepatane reversed micelles

Serralheiro et al., 1994;

1999; Prazeres et al., 1995

Continuous synthesis of kyotorphin (Tyr-Arg) catalysed by a-
chymotrypsin in an ultrafiltration cell

Flörsheimer et al., 1989;

Fischer et al., 1994

Table 6.8 Biomedical applications

Enzyme-réaction description Reference

Development of HF reactor with L-asparaginase for the
deamidation of L-asparagine for the chemotherapy of cancer

Jackson et al., 1979; Pedersen et

al., 1978a; Mazzola & Vecchio,

1980

Development of HF reactor with carboxypeptidase Cl for the
conversion of folates for the chemotherapy of cancer

Pedersen et al, 1978a

Enzymatic membrane reactors 173