Multiphase Bioreactor Design

Staining techniques associated to image analysis (IA) are becoming ubiquitous for the
examination of active regions of hyphae and physiological characterisation. The first
attempt to quantify the proportion of growing apices in Penicillium chrysogenum was

Table 2.1 Applications to fungi

Microorganism Comments Reference

Morphology characterisation Tucker et al. (1992)

Rheology Makagiansar et al. (1993)

Tucker and Thomas

(1993)

Nielsen et al (1995)

Nielsen & Krabben

(1995)

Penicillium
chrysogenum

Morphology kinetics in repeated fed-batch

Mixing; rheology

Jüsten et al. (1996)

Penicillium roqueforti Morphology classification Pichon, Vivier & Pons
(1992a)
Pellets Cox & Thomas (1992)
Rheology Olsvik et al. (1993)
Cyclone bioreactor Kamilakis & Allen
(1995)
Carbon dioxide effect Mcintyre & Mcneil
(1997a)
Mcintyre & Mcneil
(1997b)

Aspergillus niger

Fractal dimension of pellets Ryoo (1999)

Aspergillus oryzae Rheology Amanullah et al. (1999)

Mortierella alpina Morphology classification; pilot-scale Higashiyama et al.
(1999)

Fusarium moniliforme Mixing Priede et al. (1995)

Table 2.2 Applications to filamentous bacteria

Microorganism Comments Reference

Streptomyces tendae Morphology
classification

Reichl, King & Gilles

(1992)

Treskatis et al. (1997)

Streptomyces virginiae Morphology
classification

Yang et al. (1996)

Streptomyces ambofaciens Filament characterisation Pons et al. (1998)

New methodologies for multiphase bioreactors 2 31