Science - USA (2018-12-21)

of dynamin, extended coating of the tube is not
needed, and one or a few rings appear capable of
mediating scission ( 34 ). Our measurements of
scission by diffraction-limited puncta of Snf7 are
consistent with imaging in cells ( 29 ) and with
scission by rings or cones of molecular dimen-
sions. The mechanical nature of constriction and
force generation remains to be elucidated through
structural approaches.

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ACKNOWLEDGMENTS

We thank J.-Y. Lee, H. Aaron, S. Ruzin, and D. Schichnes for

assistance with imaging; M. Vahey, D. Fletcher, and P. Lishko

for advice on the aspiration pipette setup; A. Lee for assistance

with the optical trap force calibration; and C. Glick for advice

with the microfluidics.Funding:Research was supported by a

Marie Skłodowska-Curie postdoctoral fellowship smStruct

(J.S.),anNSFpredoctoralfellowship(M.R.P.),NIHgrant

R56AI127809 and R01GM032543 (C.B.), DOE grant DE-AC02-

05CH11231 (C.B.), the Howard Hughes Medical Institute

(C.B.), NIH grant R01AI112442 (J.H.H.), the Max Planck Society

(A.H.B. and G.H.), and the German Research Foundation CRC

807 (A.H.B. and G.H.).Author contributions:Conceptualization,

J.S., M.R., D.H.G, S.Y., M.R.P., A.H.B., C.B., G.H., and J.H.H;

methodology, J.S., S.Y., M.R., D.H.G, A.H.B., M.R.P., I.-H.L., and

L.-A.C.; Confleezers, J.S., S.Y., and I.-H.L., software, J.S.; formal

analysis, J.S. and A.H.B.; investigation, J.S., S.Y., M.R., M.R.P.,

A.H.B., and D.H.G; resources, J.S., S.Y., M.R., M.R.P., I.-H.L.,

L.-A.C., and X.R.; data curation, J.S; writing of original draft, J.S.

and J.H.H; manuscript review and editing, J.S., S.Y., M.R.,

M.R.P., I.-H.L, L.-A.C, A.H.B., D.H.G., X.R., G.H., C.B., and J.H.H ;

visualization, J.S.; supervision, G. H, C.B., and J.H.H.Competing

interests:The authors declare no competing interests.Data

and materials availability:All data are available in the main

text or the supplementary materials. Code is available at

https://github.com/JohSchoeneberg/Confleezers.

SUPPLEMENTARY MATERIALS

http://www.sciencemag.org/content/362/6421/1423/suppl/DC1

Materials and Methods

Figs. S1 to S4

References ( 35 – 37 )

Movies S1 and S2

1 February 2018; resubmitted 17 September 2018

Accepted 7 November 2018

10.1126/science.aat1839

Schöneberget al.,Science 362 , 1423–1428 (2018) 21 December 2018 6of6

RESEARCH | REPORT

on December 20, 2018^

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