Nature - 2019.08.29

4

nature research | reporting summary

April 2018

C2C12 (CRL-1772) and HEK293S (CRL-3022) were purchased from ATCC.

Neuroblastoma cell line (Neuro2a) was purchased from Sigma-Aldrich (89121404).

Authentication RNA-sequencing of the cell lines provide authentification.

Mycoplasma contamination All the cell lines were routinely tested for mycoplasma infection and confirmed as negative for mycoplasma contamination.

Commonly misidentified lines

(See ICLAC register)

No commonly misidentified cell line was used.

Animals and other organisms

Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research

Laboratory animals Mus musculus was used as an animal model.

The strains were as followed:

• C57BL/6J mice were obtained from the Jackson Laboratory.


• Ucp1-Cre mice (Stock No. 024670) were obtained from the Jackson Laboratory.


• Pparg flox mice (Stock No. 004584) were obtained from the Jackson Laboratory.


• Bckdhatm1a(EUCOMM)Hmgu mice were obtained from EuMMCR and used for generation of Bckdha flox mice.


• BAT-specific Bckdha-KO mice were generated by crossing Bckdha flox mouse and Ucp1-Cre mouse.


• dCas9-KRAB mice were generated by a site-specific integrase-mediated approach (TARGATT system), in which a construct
  having dCas9-KRAB was integrated into H11 locus to express dCas9-KRAB ubiquitously.


• Transgenic (Tg) mice of gRNA targeting Slc25a44 were generated by conventional transgenics.


• Slc25a44 KD mice were generated by crossing dCas9-KRAB mouse and Slc25a44-gRNA mouse.
  Adult males and females aged 8-16 weeks were used for the experiments. Littermate controls with same sex were used. All the
  mice had free access to food and water, 12 hr light cycles, and were caged at 23 C.

Wild animals This study did not involve wild animals

Field-collected samples This study did not involve samples collected from the field.

Human research participants

Policy information about studies involving human research participants

Population characteristics Thirty-three healthy young Japanese male volunteers participated in this study (23.4 ± 0.58 years old). BMI was within normal

range (21.0 ± 0.30 kg/m2).

Recruitment Volunteers were recruited by trial awareness-raising posters and consultant email-out. Potential selection biases were not

detected/observed.