Nature - 2019.08.29

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nature research | reporting summary

October 2018

Field-specific reporting

Please select the one below that is the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/documents/nr-reporting-summary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size Sample size for mouse experiments was determined by feasibility and the magnitude of the interventions impact. For the studies included in

the letter, the impact of intestinal reconstitution with lantibiotic-producing Blautia producta was large, on the order of over 1000 fold

reduction in VRE colonization, enabling comparison groups to consist of 3 or more mice. All experiments were repeated at least two times

(for a total of at least 3 separate experiments).

Data exclusions No data was excluded.

Replication Experiments were repeated at least two times to demonstrate reproducibility.

Randomization We selected samples for analyses by determining microbiota composition and also diversity. Thus, in germ free mice reconstituted with

patient derived microbiota, samples differing in lantibiotic gene abundance were matched in terms of microbiota diversity.

Blinding Blinding was not feasible for these experiments since mice receiving different treatments had to be clearly labeled and segregated during the

course of the experiment.

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Animals and other organisms

Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research

Laboratory animals We used C57BL/6 mice from Jackson laboratories. Mice were female and 6 to 8 weeks of age. Rag2-/-IL2rg-/- mice were

purchased from Taconic Laboratories.

Wild animals Provide^ details^ on^ animals^ observed^ in^ or^ captured^ in^ the^ field;^ report^ species,^ sex^ and^ age^ where^ possible.^ Describe^ how^ animals^

were caught and transported and what happened to captive animals after the study (if killed, explain why and describe method; if

released, say where and when) OR state that the study did not involve wild animals.

Field-collected samples For^ laboratory^ work^ with^ field-collected^ samples,^ describe^ all^ relevant^ parameters^ such^ as^ housing,^ maintenance,^ temperature,^

photoperiod and end-of-experiment protocol OR state that the study did not involve samples collected from the field.

Ethics oversight Identify^ the^ organization(s)^ that^ approved^ or^ provided^ guidance^ on^ the^ study^ protocol,^ OR^ state^ that^ no^ ethical^ approval^ or^

guidance was required and explain why not.

Note that full information on the approval of the study protocol must also be provided in the manuscript.