3nature research | reporting summary
October 2018- Anti-mouse IgG, HRP-linked (#7076, lot 33, 1:2,000).
From Santa Cruz Biotechnology (Santa Cruz, CA, USA):- Anti-rabbit IgG-HRP (sc-2357, multiple lots, 1:5,000).
From Bethyl Laboratories (Montgomery, TX, USA):- BRD4 (A301-985A100, lot 6, 1:30,000);
- SMC1A (A300-055A, lot 6).
From Millipore (Billerica, MA, USA):- CTCF (#07-729, lot 2887267, 1:5,000);
- SOX9 (#AB5535, lot 2847051, 1:10,000);
- H3K27me3 (#07-449, lot 2972864).
From Abcam (Cambridge, MA, USA):- ALK (phospho Y1507) (ab73996, lot GR57100-14, 1:1,000);
- BORIS (ab187163, Clone EP12204, lot GR228943-6);
- H3K27ac (ab2729, lot GR3198866-1).
From NOVUS Biologicals (Littleton, CO, USA):- BORIS (NBP2-52405, Clone 20B11, lot CRT/17/124).
From Active Motif (Carlsbad, CA, USA):- BORIS (#39851, lot 18916002, 1:3,000).
From Sigma-Aldrich (Saint Louis, MO, USA):- SIX1 (HPA001893, lot I114662, 1:4,000).
From Abbott (Abbott Park, IL, USA):- Vysis LSI N-MYC (2p24) SpectrumGreen/Vysis CEP 2 SpectrumOrange Probe (07J72-001).
Validation Validation for commercially available antibodies can be found using the links below:
- https://www.cellsignal.com/products/primary-antibodies/n-myc-antibody/9405
- https://www.cellsignal.com/products/primary-antibodies/n-myc-d4b2y-rabbit-mab/51705
- https://www.cellsignal.com/products/primary-antibodies/cleaved-parp-asp214-antibody-human-specific/9541
- https://www.cellsignal.com/products/primary-antibodies/cleaved-caspase-3-asp175-antibody/9661
- https://www.cellsignal.com/products/primary-antibodies/alk-c26g7-rabbit-mab/3333
- https://www.cellsignal.com/products/primary-antibodies/akt-pan-c67e7-rabbit-mab/4691
- https://www.cellsignal.com/products/primary-antibodies/phospho-akt-thr308-antibody/9275
- https://www.cellsignal.com/products/primary-antibodies/phospho-akt-ser473-antibody/9271
- https://www.cellsignal.com/products/primary-antibodies/p44-42-mapk-erk1-2-137f5-rabbit-mab/4695
- https://www.cellsignal.com/products/primary-antibodies/phospho-p44-42-mapk-erk1-2-thr202-tyr204-197g2-rabbit-
mab/4377 - https://www.cellsignal.com/products/primary-antibodies/s6-ribosomal-protein-5g10-rabbit-mab/2217
- https://www.cellsignal.com/products/primary-antibodies/phospho-s6-ribosomal-protein-ser235-236-91b2-rabbit-mab/4857
- https://www.cellsignal.com/products/primary-antibodies/stat3-79d7-rabbit-mab/4904
- https://www.cellsignal.com/products/primary-antibodies/phospho-stat3-tyr705-antibody/9131
- https://www.cellsignal.com/products/primary-antibodies/mdr1-abcb1-d3h1q-rabbit-mab/12683
- https://www.cellsignal.com/products/primary-antibodies/sox2-d6d9-xp-rabbit-mab/3579
- https://www.cellsignal.com/products/primary-antibodies/b-actin-antibody/4967
- https://www.cellsignal.com/products/primary-antibodies/ctcf-d1a7-xp-rabbit-mab/3417
- https://www.cellsignal.com/products/primary-antibodies/normal-rabbit-igg/2729
- https://www.cellsignal.com/products/secondary-antibodies/anti-mouse-igg-hrp-linked-antibody/7076
- https://www.scbt.com/scbt/product/mouse-anti-rabbit-igg-hrp
- https://www.bethyl.com/product/A301-985A100/BRD4+Antibody
- https://www.bethyl.com/product/A300-055A/SMC1+Antibody
- http://www.emdmillipore.com/US/en/product/Anti-CTCF-Antibody,MM_NF-07-729
- http://www.emdmillipore.com/US/en/product/Anti-Sox9-Antibody,MM_NF-AB5535
- http://www.emdmillipore.com/US/en/product/Anti-trimethyl-Histone-H3-Lys27-Antibody,MM_NF-07-449
- https://www.abcam.com/alk-phospho-y1507-antibody-ab73996.html
- https://www.abcam.com/boris-antibody-ep12204-ab187163.html
- https://www.abcam.com/histone-h3-acetyl-k27-antibody-chip-grade-ab4729.html
- https://www.novusbio.com/products/boris-antibody-20b11_nbp2-52405
- https://www.activemotif.com/catalog/details/39851/boris-ctcfl-antibody-pab
- https://www.sigmaaldrich.com/catalog/product/sigma/hpa001893?lang=en®ion=US
- https://www.molecular.abbott/sal/en-us/staticAssets/AMD-US-Oncology-and-Genetics-Catalog.pdf