Krndijaet al.,Science 365 , 705–710 (2019) 16 August 2019 2of6
Fig. 1. Epithelium continues migrating during mitotic block.(A)Top:EdU
and HU injection scheme with EdU chase time points. Bottom: Maximum
Z projections (30 to 50mm range). Dashed line in bottom panels indicates the
crypt-villus interface. Scale bars, 50mm. (B) Box-and-whisker plot corresponding
to (A). ****P< 0.0001; ns, not significant; au, arbitrary units. (C) EdU fronts
plotted as a function of time. (D) Theoretical model for epithelial renewal
during gut homeostasis. (E) Predicted cell density profiles along the crypt-villus
axis depending on the level of active migratory force. (F) Control villus—
longitudinal section. Boxed regions are shown in high magnification. Scale
bar, 40mm. (G) Cell density profile of the villus shown in (F). E-cad, E-cadherin.
(H) Model fit to average density profile. (I)HUhitreatment—longitudinal
section. Boxed regions are shown in high magnification. Scale bar, 40mm.
(J) Cell density profile of the villus shown in (I). (K) Average density profiles
for control (blue) and HUhi(red), with their theoretical predictions (lines).RESEARCH | REPORT