Krndijaet al.,Science 365 , 705–710 (2019) 16 August 2019 5of6
Fig. 4. Epithelial cell migration is driven by Arp2/3.(A) EdU pulse-
chase assays; maximum Z projections (30 to 50mm). Dashed line
indicates the crypt-villus interface. Scale bars, 100mm. (B) Top: Box-and-
whisker plot for EdU fronts. P< 0.01, **P< 0.0001. Bottom: EdU
fronts plotted against time. Control, DMSO. (C) EdU pulse-chase assays;
maximum Z projections (30 to 50mm). Tam, tamoxifen. Scale bar, 50mm.
(D) Box-and-whisker plot for EdU fronts. ****P< 0.0001. (E) CK-666
treatment—longitudinal section. Boxed regions are shown in high magni-
fication. Scale bar, 50mm. (F) Cell density profile of the villus shown in
(E). (G) Average density profiles. Control (blue, same as Fig. 1H); CK-666
(green); theoretical prediction (lines). (H) Left: Radar chart displaying
relaxation speed in HUhi- or CK-666–treated mice, along four
indicated directions. Middle: Vertex separatione(t), in function of time.
Right: Initial recoil speed, box-and-whisker plot. Control: DMSO or saline,
for CK-666 and HU, respectively. *P< 0.05. (I) Mechanical model for
epithelial migration in the small intestine.RESEARCH | REPORT