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Acknowledgements We thank J. Li for the pBIB-BASTA vector; M. R. Knight
for aequorin-expressing seeds; S. Gilroy for YC3.6-expressing seeds; M. Chen,
X. Dong and T.-p. Sun for advice on physical mapping; and T.-p. Sun, J. Pei, D.
R. McClay and P. N. Benfey for discussion and reading of the manuscript. Z.J.,
M.T., F.W., W.Z. and H. Zhang were supported in part by the Shenzhen Peacock
Innovative Research Team Program; Z.J., X.Z., L.L., X.W., F.W. and F.Y. by the
Pandeng Project of Hangzhou Normal University and Chinese NSF (31301170,
U1130304); and F.W., Y.N. and Y.X. by fellowships from the China Scholarship
Council. This work was supported by grants from NSF (IOS-1457257, IOS-
0848263) and USDA (CSREES-2006-35100-17304) to Z.-M.P.; National
Key Project for Synthetic Biology (2018YFA0902500) to Z.H.; and DOE (DE-
SC0014077) to T.V.-D. and Z.-M.P.Author contributions Z.J., F.Y., Y. Xiang and Y.N. conducted genetic screens. Z.J.,
X.Z., F.Y., L.L., F.L., Y. Xue, F.W., X.W. and H. Zhao performed map-based cloning.
C.L., R.Y., F.Y. and B.B. generated T-DNA-mutagenized Arabidopsis populations.
Z.J., L.L., F.W., M.T., C.H., H. Zhao, C.P. and W.Z. carried out physiological analyses.
Z.J., M.T., W.Z., and Z.H. analysed GIPC content and ion content. M.T., Z.J., W.Z.,
and Z.H. analysed ion binding to GIPCs. Z.J., C.P. and F.W. conducted zeta
potential measurement. Z.J., L.L., H.-N.W., B.M.C., D.M.J., G.B.S., H. Zhang and
T.V.-D. participated in Ca^2 +-imaging analyses. Z.-M.P. designed and supervised
the overall research with input from Z.H., Z.J. and J.N.S. Z.-M.P., Z.J. and J.N.S.
wrote the paper. All authors discussed the results and commented on the
manuscript.Competing interests The authors declare no competing interests.Additional information
extended data is available for this paper at https://doi.org/10.1038/s41586-
019-1449-z.
supplementary information is available for this paper at https://doi.org/
10.1038/s41586-019-1449-z.
Correspondence and requests for materials should be addressed to Z.H. or
Z.-M.P.
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