Nature - 15.08.2019

reSeArCH Letter

5.1

(Å)

4.8

4.6

4.3

3.9

b) c)

d)

a)

SidJ

Human

apo CaM

b)

Resolution (1/Å)

Fourier shell correlation (FSC

)

.

SUM

HALF1 (FSCwork)

HALF2 (FSCfree)

FSC=0.5

d) SidJ739-772 SidJ628-654 CaM66-77

Extended Data Fig. 8 | Cryo-EM model refinement. a, FSC between
model and the map and cross-validation of the model fitting.
FSC0.5 = 4.3 Å for the model versus map (sum). Half-map cross validation
procedure^31 does not show overfitting in the refined model. b, Local

resolution analysis (using Relion) shows variation in the map resolution

ranging from 3.9 to 5.1 Å. c, Overview of the model fitting into the map in

the same orientation as in b. d, An example of the cryo-EM map quality

with the atomic model fitted in, showing clear density for side chains.