reSeArCH Letter
extended data table 1 | Methionine concentrations in plasma, tumour and liver across mouse models and humans
Tissues were collected at the end of each experiment. Concentrations in tissues were estimated in μM, assuming that 1 g wet tissue weight = 1 ml. Quantification was performed by using^13 C-labelled
standards for each amino acid, which were added before extraction. Cyclic loess normalization and linear regression were applied in quantification of methionine in samples without^13 C-labelled
standards. Values are mean ± s.d. n = 8 for CRC119, CRC240, CRC119 (vehicle) and CRC119 (5-FU), 7 for sarcoma on the control diet, 6 for sarcoma on the methionine-restricted diet, 7 for sarcoma
(radiation), 5 for C57BL/6J mice and 6 for humans. *P < 0.05, by two tailed Student’s t-test between the control diet and the methionine-restricted diet; #P < 0.05, by two tailed Student’s t-test
between the control diet and the methionine-restricted diet + radiation.