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ACKNOWLEDGMENTS
We thank N. Klena for critical reading of the manuscript and
M. Braun for helpful discussions. We thank N. Olieric for initially
providing the subtilisin enzyme. We thank the BioImaging Center of
the University of Geneva.Funding:This work was supported
by ERC StG 715289 (ACCENT), granted to P.G., and V.H., P.G.,
and M.L.G. are supported by Swiss National Science Foundation
(SNSF) PP00P3_157517. E.S. and S.B. are supported by the
University of Geneva. Z.L. is supported by the Czech Science
Foundation (18-08304S), the project BIOCEV (CZ.1.05/1.1.00/
02.0109) from the ERDF, and CAS (RVO: 86652036). I.Z. is
supported by GAUK (1372218). We acknowledge the Centre of
Imaging Methods core facility, Faculty of Science, Charles University,
supported by the MEYS CR (LM2015062 Czech-BioImaging).
Author contributions:M.S.-C. prepared the samples, analyzed the
cryo-EM data, and set up the immunofluorescence, Coomassie,
and Western blot experiments. M.L.G. performed subtomogram
averaging. I.Z. and Z.L. performed and analyzed the TIRF
experiments. R.A. and L.M. performed the molecular modelinganalysis. D.D. provided access and assistance with the cryo-EM F20
microscope. E.S. prepared samples for the cryo-EM session and
performed immunofluorescence experiments. S.B. prepared the SDS–
polyacrylamide gel electrophoresis gels and Western blots. P.G.
performed the cryo-EM. P.G., Z.L., L.M., and V.H. designed, analyzed,
and supervised the work, and P.G., V.H., L.M., M.S.-C., and Z.L.
wrote the manuscript.Competing interests:The authors declare
no competing interests.Data and materials availability:All data
are available in the main text or the supplementary materials.SUPPLEMENTARY MATERIALS
http://www.sciencemag.org/content/363/6424/285/suppl/DC1
Materials and Methods
Figs. S1 to S5
References ( 19 – 32 )
Movies S1 to S5
30 August 2018; accepted 19 December 2018
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