Gaoet al.,Science 363 , eaau8302 (2019) 18 January 2019 5of16
Fig. 3. Characterizing dendritic spine morphologies and postsynaptic
Homer1 across the mouse primary somatosensory cortex.(A) Coronal
MIP of a 1900- by 280- by 70-mm tissue section spanning the pia to the
white matter of the primary somatosensory cortex of a Thy1-YFP mouse
(Movie 4), additionally immunostained against Bassoon and Homer1.
Boxes denote seven regions for quantitative morphological analysis of
dendritic spines. Scale bar, 100mm. (B) (Top) Magnified MIPs of YFP-
expressing neurons in four of the regions from (A), with (bottom) further
magnified subregions showing differing spine morphologies. Scale bars,
(top) 50mm and (bottom) 10mm. (C) Scatter plots and histograms
indicating relationships between (top) spine backbone length and head
diameter and (bottom) spine neck length and neck diameter in the four
regions from (B) (figs. S13 to S15 and movie S2). (D) Two adjacent layer V
pyramidal neurons selected within the volume (magenta), one exhibiting
strong Homer 1 expression (neuron 1) and the other exhibiting weak
expression (neuron 2). (Insets) Homer1 localization or lack thereof at
apical dendritic spines (fig. S17). Scale bars, 50mm and (insets) 10mm.
(E) (Top) MIP of the local density of Homer1 puncta across a ~25-mm-thick
coronal slab, and (bottom) the cumulative number of puncta in 50- by
50- by 25-mm subvolumes across the cortex.RESEARCH | RESEARCH ARTICLE
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