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ACKNOWLEDGMENTS

This article is dedicated to Hendrik Herrmann. We thank L. Tilley

for suggestions of the timing of Kelch13 KS induction for RSAs.

We thank Jacobus Pharmaceuticals for supplying WR99210. DSM1

(MRA-1161) was received from MR4/BEI Resources, NIAID, NIH.

Funding:E.J., S.F., A.B.S., and T.S. acknowledge funding by the

Research Training Group (GRK 1459) of the German Research

Foundation (DFG). S.Schm. acknowledges funding by the German

Center for Infection Research (TTU 03.806), M.S. thanks the

Jürgen Manchot Stiftung and S.Scha. the Claussen-Simon Stiftung

for funding. W.A.M.H. obtained financial support from Netherlands

Organization for Scientific Research (NWO-VENI, 722.012.004).

Author contributions:J.B. designed research, carried out

experiments, analyzed data, prepared figures, and wrote the

methods section. S.Scha., S.Schm., E.J., and S.F. designed and

carried out experiments, analyzed data, and prepared figures.

M.S., R.S., B.B., U.F., P.M.-R., and A.B.S. carried out experiments.

W.A.M.H. and C.G.T. conducted the mass spectrometry

experiments, analyzed data, and prepared figures. R.B. supervised

the mass spectrometry experiments and analyzed data. T.S.

conceived of and supervised the project, designed experiments,

carried out imaging and image analysis, prepared figures, and

wrote the paper with critical input from all authors.Competing

interests:The authors declare no competing interests.Data and

materials availability:All data are available in the manuscript

or the supplementary materials, and the Kelch13, Eps15, and CHC

mass spectrometry data are available via ProteomeXchange with

identifier PXD008219.

SUPPLEMENTARY MATERIALS

science.sciencemag.org/content/367/6473/51/suppl/DC1

Materials and Methods

Fig. S1 to S14

Table S1

References ( 54 – 78 )

Data S1 to S4

View/request a protocol for this paper fromBio-protocol.

26 March 2019; resubmitted 19 September 2019

Accepted 30 October 2019

10.1126/science.aax4735

Birnbaumet al.,Science 367 ,51–59 (2020) 3 January 2020 9of9

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