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Acknowledgements We thank all members of the Ginty laboratory for discussions and critical
feedback during the course of this work. We thank L. Yap, A. Rodrigues, A. Shyer, B. Shrestha,
C. Santiago, C. Harwell, D. Paul, G. Fishell, L. Orefice, L. Goodrich, M. Pecot, and R. Wolfson for
feedback and critical evaluation of the data and manuscript. We thank L. Yap and M.
Greenberg for providing the base construct for AAV-mediated shRNA delivery. We thank M.
Greenberg for access to the NextSeq 500 sequencing platform. This work was supported by
NIH grant NS97344 (D.D.G.), Howard Hughes Medical Institute–Life Sciences Research
Foundation postdoctoral fellowship (D.E.W.), NIH grant 1K99GM121852 (D.E.W.), NIH grant
5R33CA212697 (A.M.K.), the Bertarelli Foundation (D.D.G.), a Fix Fund Postdoctoral Fellowship
(N.S.), and the Edward R. and Anne G. Lefler Center for Neurodegenerative Disorders. D.D.G. is
an investigator of the Howard Hughes Medical Institute.Author contributions N.S. and D.D.G. conceived and designed the project. N.S. designed,
executed and analysed all experiments with assistance and guidance from D.E.W. and A.M.K.
on the STITCH/SPRING analysis. N.S., K.F. and K.L. designed, prepared, and validated AAV
constructs. N.S. and D.D.G. wrote the manuscript with input from all authors.
Competing interests The authors declare no competing interests.Additional information
Supplementary information is available for this paper at https://doi.org/10.1038/s41586-019-
1900-1.
Correspondence and requests for materials should be addressed to D.D.G.
Peer review information Nature thanks Jeremy Dasen and the other, anonymous, reviewer(s)
for their contribution to the peer review of this work.
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