Extended Data Fig. 4 | Endogenous GPR174 does not affect the follicular
distribution of naive B cells or the accumulation of freshly activated B cells
to the T-cell–B-cell border. a, Splenic distribution of GPR174-sufficient and
-deficient naive B cells 24 h after adoptive transfer. WT, wild type; KO,
knockout. Data are representative of two independent experiments involving
either male or female cells. Scale bars, 50 μm. b, Left, splenic distribution of
GPR174-sufficient or -deficient B cells that were stimulated with anti-IgM, 6 h
after adoptive transfer. Scale bar, 50 μm. Middle, definitions of follicular area,
the T-cell–B-cell border and the T-cell zone, based on IgD and CD3 staining.
Right, relative abundance of transferred B cells in the indicated regions. Data
(n = 24 biological replicates) represent two independent experiments with
similar results. Lines in scatter plots denote mean. We used two-way ANOVA
with Bonferroni’s multiple comparisons; ns, not significant.