Extended Data Fig. 9 | NBPF family genes in the human hippocampus.
a, Expression of NBPF family genes shown using the same layout as in Fig. 1b.
Grey, no expression; blue, relative expression. b, Domains of NBPF1.
c, Evolutionary history inferred using the neighbour-joining method. The tree
is drawn to scale, with branch lengths in the same units as those of the
evolutionary distances used to infer the phylogenetic tree. The evolutionary
distances were computed using the Poisson correction method and are in the
units of the number of amino acid substitutions per site. The analysis involved
six amino acid sequences. Evolutionary analyses were conducted in MEGA X.
d, Overexpression of NBPF1 promotes DG formation at E13.5, and is observed at
E15.5 in mouse. Scale bars, 500 μm. The experiment was repeated six times
independently with similar results. e, Scheme depicting the position in the
mouse brain at E18.5 of the slice in f. f, Overexpression of NBPF1 promotes DG
formation at E13.5, and this is observed at E18.5 in mouse. Scale bars, 1,000 μm.
The experiment was repeated six times independently with similar results.
g, Flow chart of patch-qRT–PCR. h, Relative expression of specific genes of
GFP+ cells. **P = 0.0020, *P = 0.0408, two-sided t-test; n = 10 GFP cells; 8 NBPF1–
GFP cells. Mean ± s.e.m. i, Normalized ATAC-seq profiles of PROX1 in GW25
hippocampus with three independent biological replicates (Rep1, Rep2 and
Rep3) showing the activation of PROX1. The amplifying panel shows the
predicted LHX2 binding sites.