Article
Extended Data Fig. 6 | Z-series of a 14-d.p.f. embryo with a PSA. This figure is
related to Fig. 3. Series of confocal z-sections of the embryo stained for T
(green), OCT4 (red) and N-cadherin (grey), showing formation of PSA. The
thickness of every section was 12 μm. Numbers on the left indicate the number
of sections. Red and white arrows indicate T+ and N-cadherin+ cells,
respectively. Some T+ cells (red arrows) in the 13# section located in the amnion
epithelium, consistent with the distribution of some T+ cells in the monkey
amnion epithelium^23. T+ cells (red arrows) in the 16# section disrupted the
N-cadherin-forming basement-membrane barriers between epiblast and
hypoblast and focally migrated from the embryonic disc to generate the PSA.
Similar data were observed in 3 out of 3 embryos from two experiments. Scale
bars, 50 μm.