Extended Data Fig. 7 | Z-series of a 14-d.p.f. embryo with a cell emigration
region. This figure is related to Fig. 3. Series of confocal z-sections of the
embryo stained for GATA6 (green), OCT4 (red) and T (grey). The thickness of
every section was 12 μm. Numbers on the left indicate the number of sections.
Red and white arrows denote T+G ATA6+O C T4− cells and T+G ATA6+O C T4+ cells,
respectively. In the 7# and 8# sections, some migrating T+ EPIs invaded the
space near the visceral endoderm and co-expressed GATA6 (white arrows).
Similar data were observed in 4 out of 5 embryos from three experiments. Scale
bars, 50 μm.