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nature research | reporting summary
October 2018(R&D Systems, AF2085, polyclonal, KQP0617031, 1:200), goat anti FLK-1 (KDR) (R&D Systems, AF357, polyclonal, CVE0617081,
1:100) and WGA Alexa Fluor® 647 (Invitrogen, W32466, direct labeled, 1988457, 1:500)Validation All the antibodies have been validated by the companies from which they were offered. This information was used for further
validate of the antibodies used in this work. Details of the validation statements, antibody profiles and relevant citations can be
found on the manufacturer's website.
All the antibodies used in this work are for immunostaining purpose only. We list the immunostaining validation of the
manufactures and the number of citations as follows:
- Oct3/4 (SC5279): https://www.scbt.com/p/oct-3-4-antibody-c-10
Oct3/4 (SC5279) antibody was validated by the manufacturer using glandular cells and mouse embryos. We verified that this
antibody stains the nuclei of ICM and EPIs, as expected. More than 1303 citations. - Brachyury(T)(SC20109): https://www.scbt.com/zh/p/brachyury-antibody-h-210
Brachyury(T)(SC20109) antibody was validated by the manufacturer using human lung tissue. We verified that this antibody
stains the nuclei of posterior EPIs, as expected. More than 9 citations. - Sox2 (AB5603): http://www.merckmillipore.com/CN/zh/product/Anti-Sox2-Antibody,MM_NF-AB5603
Sox2 (AB5603) antibody was validated by the manufacture using H9 human stem cells. We verified that this antibody stains the
nuclei of EPIs, as expected. More than 166 citations. - Sox17 (AF1924): https://www.rndsystems.com/cn/products/human-sox17-antibody_af1924
Sox17 (AF1924) antibody was validated by the manufacture using B16 mouse cell line and human BG01V cells. We verified that
this antibody stains the nuclei of morula and PrEs, as expected. More than 123 citations. - Klf 4 (09-821): http://www.merckmillipore.com/CN/zh/search/09-821
Klf 4 (09-821) antibody was validated by the manufacture’s using NIH/3T3, A431, and HeLa cells. We verified that this antibody
stains the nuclei of early EPIs. More than 4 citations. - Klf17(HPA024629):https://www.atlasantibodies.com/products/antibodies/primary-antibodies/triple-a-polyclonals/klf17-
antibody-hpa024629
Klf 17 (HPA024629) antibody was validated by the manufacture using human testis and tonsil tissues. We verified that this
antibody stains the nuclei of early EPIs. More than 6 citations. - β-Catenin (AB32572): https://www.abcam.cn/beta-catenin-antibody-e247-chip-grade-ab32572.html
β-Catenin (AB32572) antibody was validated by the manufacture using A431 and wild-type HAP1 cells. We verified that this
antibody stains the membrane of the embryo cells. More than 335 citations. - E-cadherin (AB76055): https://www.abcam.cn/e-cadherin-antibody-m168-c-terminal-ab76055.html
E-cadherin (AB76055) antibody was validated by the manufacture using A431 cells. We verified that this antibody stains the
membrane of the embryo cells which N-cadherin did not expressed. More than 137 citations. - Cytokeratin7 (CK7) (AB181598) https://www.abcam.cn/cytokeratin-7-antibody-epr17078-cytoskeleton-marker-ab181598.html
Cytokeratin7 (CK7) (AB181598) antibody was validated by the manufacture using A549 cells. We verified that this antibody stains
the membrane of trophoblast. More than 24 citations. - N-cadherin (AB12221): https://www.abcam.cn/n-cadherin-antibody-ab12221.html
N-cadherin (AB12221) antibody was validated by the manufacture using mouse differentiated embryonic stem cells. We verified
that this antibody stains the membrane of mesenchymal cells. More than 84 citations. - Otx2 (SC514195): https://www.scbt.com/zh/p/otx2-antibody-d-8
Otx2 (SC514195) antibody was validated by the manufacture using Jurkat, Hep G2, Hela nuclear extract and hES differerntiated
cells in our lab. We verified that this antibody stains the nuclei of parts of hypoblast cells. More than 2 citations. - Lefty1 (AF746): https://www.rndsystems.com/cn/products/human-mouse-lefty-antibody_af746
Lefty1 (AF746) antibody was validated by the manufacture using mouse ulters in the citation. We verified that this antibody
stains cytoplasma AVE cells, as expected. More than 1 citations. - Sox1 (AF3369): https://www.rndsystems.com/cn/products/human-mouse-rat-sox1-antibody_af3369
Sox1 (AF3369) antibody was validated by the manufacture using ectoderm differentiated BG01V human embryonic stem cells.
We verified that this antibody stains neural stem cells in our lab. More than 30 citations. - Pax6 (901301): https://www.biolegend.com/en-us/products/purified-anti-pax-6-antibody-11511
Pax6 (901301) antibody was validated by the manufacture using frozen human iPSC derived neural rosettes. We verified that this
antibody stains neural stem cells in our lab. More than 97 citations. - Laminin (L9393): https://www.sigmaaldrich.com/catalog/product/sigma/l9393
Laminin (L9393) antibody was validated by the manufacture using human Tongue sectons. We verified that this antibody stains
basement membrane, as expected. More than 776 citations. - Tead4(HPA056896):https://www.atlasantibodies.com/products/antibodies/primary-antibodies/triple-a-polyclonals/tead4-
antibody-hpa056896/
Tead4(HPA056896) antibody was validated by the manufacture using human A431 cells. We verified that this antibody stains the
nuclei of parts of EPIs and TEs, as expected. More than 4 citations. - Foxa2 (SC6554): https://www.scbt.com/zh/p/hnf-3beta-antibody-m-20
Foxa2 (SC6554) antibody was validated by the manufacture using HepG2 cells. We verified that this antibody stains the nuclei of
neural stem cells in our lab. More than 66 citations. - Oct3/4 (SC8628): https://www.scbt.com/zh/p/oct-3-4-antibody-n-19
Oct3/4 (SC8628) antibody was validated by the manufacturer using mouse embryos. We verified that this antibody stains the
nuclei of ICM and EPIs, as expected. More than 79 citations. - hCG (AB9582): https://www.abcam.cn/hcg-beta-antibody-5h4-e2-ab9582.html
hCG (AB9582) antibody was validated by the manufacturer using human chorionic villus cells. We verified that this antibody
stains the AME, as expected. More than 8 citations. - PRDM14 (AB4350): http://www.merckmillipore.com/CN/zh/product/Anti-PRDM14-Antibody,MM_NF-AB4350#anchor_REF
PRDM14 (AB4350) antibody was validated by the manufacturer using human lung tissue lysate. We verified that this antibody
stains the early EPIs, as expected. More than 1 citations. - TFCP2L1 (AF5726): https://www.rndsystems.com/cn/products/human-tfcp2l1-antibody_af5726
TFCP2L1 (AF5726) antibody was validated by the manufacturer using human placental tissue. We verified that this antibody
stains the early EPIs and naïve stem cells in our lab. More than 5 citations. - NANOG (AF1997): https://www.rndsystems.com/cn/products/human-nanog-antibody_af1997