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Extended Data Fig. 6 | Single-turnover cA 4 cleavage by SIRV1 gp29 and
variants, and chemical rescue with imidazole. a, Phosphorimage of TLC
visualizing cA 4 cleavage by SIRV1 gp29 H47A (4 μM dimer, 50 °C) in the presence
or absence of 500 mM imidazole, over time. The rate of cA 4 cleavage to
generate A 2 >P and A 2 -P was calculated by quantifying densiometric signals
from the phosphorimage (n = 3 technical replicates). b, Plot comparing the
single-turnover rates of cA 4 by SIRV1 gp29, its E88A variant and its H47A variant,
in the presence or absence of imidazole. Cleavage of cA 4 by the H47A variant
can be partially restored when the reaction is supplemented with 500 mM
imidazole. Data are mean and s.d. (n = 3 technical replicates). For gel source
data, see Supplementary Fig. 1.