Extended Data Fig. 2 | H2A.Z interacts with ORC1 in an H4K20me2-
dependent manner. a, b, High-performance liquid chromatography (HPLC) of
unmodified H4 peptide and H4K20me2 peptide in H2A (a) and H2A.Z (b)
nucleosomes. c, Western blot analysis showing the signal of SUV420H1 and
H4K20me1/2/3 in shGFP cells or shSUV420H1 cells with stably expressed Flag-
tagged H2A or H2A.Z. d, Real-time PCR analysis shows the level of expression of
SUV420H1 and SUV420H2 in cells transfected with shRNA targeting GFP,
SUV420H1 or SUV420H2. The expression level was normalized to that of
GAPDH. e, Left, enrichment of ORC1 on H2A or H2A.Z mononucleosome from
wild-type or SUV420H2-knockdown cells. Right, enrichment of ORC1 on H2A.Z
mononucleosomes from wild-type or SUV420H1/2-knockdown cells. Data in
panel d are means ± s.e.m.; n = 3 biological replicates; two-tailed unpaired t-
test. The experiment in panel a was independently repeated four times with
similar results. The experiments in panels c, e were independently repeated
twice with similar results. H4 was used as a loading control and sample
processing control in c and e, respectively. For gel source data, see
Supplementary Fig. 1.