Extended Data Fig. 5 | H2A.Z regulates H4K20me2 and ORC1 on a genome-
wide level. a, Box plot showing the dynamics of H4K20me2 at H4K20me2 peak
regions (n = 99, 574) after knocking down both SUV420H1 and SUV420H2.
b, Venn diagram showing the overlap between H4K20me1, H4K20me2 and
H4K20me3 peaks genome wide. c, Heat maps and corresponding box plots
showing the dynamics of H4K20me1, H4K20me2 and H4K20me3 at 10-kilobase
regions around the centres of the H4K20me2 peaks (n = 99, 574). d, e, Dot plot
showing a positive correlation between H2A.Z and H4K20me2 (d) and H2A.Z
and ORC1 (e) at H2A.Z peaks (n = 58,6 42). r, Pearson’s correlation coefficient.
f, Mass-spectrometry analysis of chromatin H4K20me2 abundance after H2AFZ
knockdown. g, Western blot analysis of ORC1, SUV420H1, MCM2, H4K20me1,
H4K20me2 and H4K20me3 in the chromatin fraction from cells arrested at G1
phase. h, Box plot showing the nascent-strand (NS) signal of RNase-treated and
untreated samples from the NS peaks (n = 41, 8 50). i, j, Dot plot showing the
dynamics of H4K20me2 (i; n = 99, 574) and ORC1 (j; n = 100,917) after knocking
down H2AFZ. k, Genome-wide distribution of plus-ZD-ORC1 peaks (n = 20,978).
UTR, untranslated region. Data in panels a, c, h were analysed by two-tailed
Wilcoxon test. Data in panel f are means (n = 2 biological replicates) with dot
plots overlaid. Data in panels i, j were analysed by one-sided Fisher’s exact test
without adjustments for multiple comparisons. The western blots in panel g
were independently repeated twice with similar results, and H4 was used as a
loading control and sample processing control. For the box plots in panels a,
c, the centre lines represent the medians, the box limits are the 25th and 75th
percentiles, and the whiskers are the minimum to maximum values. For the
violin plots in panel h, the centres of the boxes represent the medians, the box
limits are the 25th and 75th percentiles, and the upper and lower limits show the
95% confidence interval. For gel source data, see Supplementary Fig. 1.