Extended Data Fig. 7 | H2A.Z regulates early-replication origins through
SUV420H1. a, FACS analysis of cell-cycle progression for siNC and siH2A.Z cells
released from G1/S arrest. b, Statistical results from panel a. To quantify cell-
cycle progression, we normalized the peak DAPI signal of all time points to the
peak DAPI signal at 0 h (G1/S arrest). We defined the DAPI signal at the G1/S
boundary as value 1; when cells entered G2/M phase, the value is near 2.
c, Western blot analysis of H4K20me levels in SUV420H1-knockdown cells
rescued by wild-type or mutant (R257A, K333A) SUV420H1. d, H4K20me2, NS
and BrdU levels at early origins (targets 1, 3) and late origins (target 2) in
SUV420H1-knockdown (‘Sh’) cells rescued by wild-type or mutant SUV420H1.
Data in panels b, d are means ± s.e.m.; n = 4 biological replicates in b; n = 3
biological replicates in d; two-tailed unpaired t-test. The FACS experiment in
panel a was independently repeated four times with similar results. The
western blots in panel c were independently repeated three times with similar
results. H4 was used as a loading control and sample processing control in
panel c. For gel source data, see Supplementary Fig. 1. For the FACS gating
strategy, see Supplementary Fig. 3.