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nature research | reporting summary
October 2018CD25 PE (Clone: PC61.5; Cat. 12-0251; Lot: 4277529; eBioscience)
CD44 APC/Cy7 (Clone: IM7; Cat. 103028; Lot: B262797; Biolegend)
CD62L AF700 (Clone: MEL-14; Cat. 56-0621-821; Lot: 4306534; eBioscience)
Anti-mouse CD3ε (Clone: 145-2C11; Cat. 100314 ; Lot:B267827; Biolegend)
Anti-mouse CD28 (Clone: 37.51; Cat. 102112; Lot:B261983; Biolegend)Validation all antibodies used are commonly used in the field and have been validated in previous publications/by the manufacturer.
References and manufacturer validations can be found here:
Anti-H2A.Z (Abcam ab4174): https://www.abcam.com/histone-h2az-antibody-chip-grade-ab4174.html#top-496
Anti-H4 (Millipore #05858): https://www.merckmillipore.com/CN/zh/product/Anti-Histone-H4-Antibody-pan-clone-62-141-13-
rabbit-monoclonal,MM_NF-05-858?ReferrerURL=https%3A%2F%2Fwww.google.com%2F&bd=1
Anti-H3 (Cell signaling #9715): https://www.cellsignal.com/products/primary-antibodies/histone-h3-antibody/9715
Anti-H4K20me1 (Abcam ab9051): https://www.abcam.com/histone-h4-mono-methyl-k20-antibody-chip-grade-ab9051.html
Anti-H4K20me2 (Abcam ab9052): https://www.abcam.com/histone-h4-di-methyl-k20-antibody-chip-grade-ab9052.html
Anti-H4K20me3 (Abcam ab9053): https://www.abcam.com/Histone-H4-tri-methyl-K20-antibody-ChIP-Grade-ab9053/
reviews/45378
Anti-H3K36me3 (Abcam Ab9050): https://www.abcam.com/Histone-H3-tri-methyl-K36-antibody-ChIP-Grade-ab9050/
reviews/61366
Anti-ORC1 (Abcam ab60): https://www.abcam.com/orc1-antibody-7f61-chip-grade-ab60.html
Anti-PCNA (Abcam ab29): https://www.abcam.com/pcna-antibody-pc10-ab29.html
Anti-BrdU (BD Biosciences BD44): https://www.bdbiosciences.com/us/applications/research/apoptosis/purified-antibodies/
purified-mouse-anti-brdu-b44/p/347580
Anti-Flag (Sigma F7452): https://www.sigmaaldrich.com/catalog/product/sigma/f7425?lang=zh®ion=CN
Anti-ORC2 (Abcam ab68348): https://www.abcam.com/orc2-antibody-ab68348.html
Anti-MCM2 (Abcam ab4461): https://www.abcam.com/mcm2-antibody-ab4461.html
Anti-RPA (Abcam ab79398): https://www.abcam.com/rpa70-antibody-epr3472-ab79398.html
Anti-Suv420H1 (Novus, NBP1-78303): https://www.novusbio.com/products/suv420h1-antibody_nbp1-78303
Anti-Tubulin (Abcam Ab6046): https://www.abcam.com/beta-tubulin-antibody-loading-control-ab6046.html
Anti-His (Invitrogen MA1-21315): https://www.fishersci.com/shop/products/anti-6x-his-epitope-tag-clone-his-h8/MA121315?
gclid=Cj0KCQjwoKzsBRC5ARIsAITcwXEHNjo-RWL5Ce9YPTuOXqXAPKmQjn-
y3MVTyALYYMfJCpWgli4DGwkaAmT7EALw_wcB&ef_id=Cj0KCQjwoKzsBRC5ARIsAITcwXEHNjo-RWL5Ce9YPTuOXqXAPKmQjn-
y3MVTyALYYMfJCpWgli4DGwkaAmT7EALw_wcB:G:s&cid=SEM_GAW_20190909_CB88IN&ppc_id=FisherSciNonbrand_goog_649
2004464_80722830231_AntibodiesDSA_b_381598720312_791621048115855394&s_kwcid=AL!4428!3!381598720312!b!!g!!
Anti-CD3-FITC (eBioscience 11-0031-82 clone 145-2C11): https://www.thermofisher.com/cn/zh/antibody/product/CD3e-
Antibody-clone-145-2C11-Monoclonal/11-0031-82
Anti-CD4-PerCp-Cy5.5 (eBioscience 45-0042 clone RM4-5): https://www.thermofisher.com/cn/zh/antibody/product/CD4-
Antibody-clone-RM4-5-Monoclonal/35-0042-82
Anti-CD8-APC (Biolegend 17-0081 clone 53-6.7): https://www.thermofisher.com/cn/zh/antibody/product/CD8-alpha-Antibody-
clone-5H10-Monoclonal/MCD0805
Anti-CD44-APC/Cy7 (Biolegend 103028): https://www.biolegend.com/en-us/products/apc-cy7-anti-mouse-human-cd44-
antibody-3933
Anti-CD62L-AF700 (eBioscience 56-0621-821 Clone MEL-14): https://www.thermofisher.com/cn/zh/antibody/product/CD62L-L-
Selectin-Antibody-clone-MEL-14-Monoclonal/56-0621-82
Anti-B220-PE (eBioscience 12-0452 clone RA3-6B2): https://www.thermofisher.com/antibody/product/12-0452-85.html?
CID=AFLCA-12-0452-85
Anti-CD25-PE (eBioscience 12-0251-82): https://www.thermofisher.com/cn/zh/antibody/product/CD25-Antibody-clone-PC61-5-
Monoclonal/12-0251-82
Live/Dead (invitrogen L34959): https://www.thermofisher.com/order/catalog/product/L34959
Anti-mouse CD28 (Biolegend 102112): https://www.biolegend.com/en-us/products/purified-anti-mouse-cd28-antibody-117
Anti-mouse CD3 (Biolegend 100314): https://www.labome.com/product/BioLegend/100314.htmlEukaryotic cell lines
Policy information about cell lines
Cell line source(s) HeLa cell line was originally obtained from ATCC.Authentication Identity of HeLa cell line was frequently checked by the morphological features, but not authenticated.Mycoplasma contamination The cell lines were regulary tested for mycoplasma contamination. And if have contamination, we immediately treated cell
lines with plasmocin(Invivogen).Commonly misidentified lines
(See ICLAC register)No commonly misidentified cell lines were used.Animals and other organisms
Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research
Laboratory animals H2A.Z flox mice were purchased from Riken Bioresource Center(RBRC05765). CD4-Cre transgenic mice were purchased from The
Jackson Laboratory. H2A.Zflox/flox CD4-Cre mice were generated through crossing H2A.Zflox/flox mice to CD4-Cre transgenic
mice. All mice were housed under specific pathogen-free conditions in the animal care facilities at the Institute of Biophysics,
Chinese Academy of Sciences.