Nature - USA (2020-01-23)

4

nature research | reporting summary

October 2018

All mice used for experiments were 6-10 weeks-old. Age and sex matched female or male mice were used for each experiment.

Wild animals No wild animals were involved.

Field-collected samples No field-collected samples were used.

Ethics oversight All animal experiments were performed in accordance with the guidelines of the Institute of Biophysics, Chinese Academy of

Sciences, using protocols approved by the Institutional Laboratory Animal Care and Use Committee.

Note that full information on the approval of the study protocol must also be provided in the manuscript.

ChIP-seq

Data deposition

Confirm that both raw and final processed data have been deposited in a public database such as GEO.

Confirm that you have deposited or provided access to graph files (e.g. BED files) for the called peaks.

Data access links

May remain private before publication.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE134988

Files in database submission GSM3983183 hela-siNC-H2A.Z_rep1

GSM3983184 hela-siNC-H2A.Z_rep2

GSM3983185 hela-siH2A.Z-H2A.Z_rep1

GSM3983186 hela-siH2A.Z-H2A.Z_rep2

GSM3983187 hela-siNC-H4K20me1_rep1

GSM3983188 hela-siNC-H4K20me1_rep2

GSM3983189 hela-siNC-H4K20me2_rep1

GSM3983190 hela-siNC-H4K20me2_rep2

GSM3983191 hela-siNC-H4K20me3_rep1

GSM3983192 hela-siNC-H4K20me3_rep2

GSM3983193 hela-siNC-native-input

GSM3983194 hela-siH2A.Z-native-input

GSM3983195 hela-siH2A.Z-H4K20me1_rep1

GSM3983196 hela-siH2A.Z-H4K20me1_rep2

GSM3983197 hela-siH2A.Z-H4K20me2_rep1

GSM3983198 hela-siH2A.Z-H4K20me2_rep2

GSM3983199 hela-siH2A.Z-H4K20me3_rep1

GSM3983200 hela-siH2A.Z-H4K20me3_rep2

GSM3983201 hela-siNC-ORC1_rep1

GSM3983202 hela-siNC-ORC1_rep2

GSM3983203 hela-siH2A.Z-ORC1_rep1

GSM3983204 hela-siH2A.Z-ORC1_rep2

GSM3983205 hela-siNC-NS_rep1

GSM3983206 hela-siNC-NS_rep2

GSM3983207 hela-siNC-Rnase-treated-NS_rep1

GSM3983208 hela-siNC-Rnase-treated-NS_rep2

GSM3983209 hela-siNC-genome

GSM3983210 hela-siH2A.Z-NS

GSM3983211 hela-siH2A.Z-Rnase-treated-NS

GSM3983212 hela-siH2A.Z-genome

GSM3983213 hela-siNC-BrdU-10min_rep1

GSM3983214 hela-siNC-BrdU-10min_rep2

GSM3983215 hela-siNC-BrdU-1h

GSM3983216 hela-siNC-BrdU-3h

GSM3983217 hela-siNC-BrdU-6h

GSM3983218 hela-siH2A.Z-10min_rep1

GSM3983219 hela-siH2A.Z-10min_rep2

GSM3983220 hela-siH2A.Z-1h

GSM3983221 hela-siH2A.Z-3h

GSM3983222 hela-siH2A.Z-6h

GSM3983223 hela-siNC-RNA-seq_rep1

GSM3983224 hela-siNC-RNA-seq_rep2

GSM3983225 hela-siH2A.Z-RNA-seq_rep1

GSM3983226 hela-siH2A.Z-RNA-seq_rep2

GSM3983227 mouse-active-T-cell-siNC-H4K20me2

GSM3983228 mouse-active-T-cell-siH2A.Z-H4K20me2

GSM3983229 mouse-active-T-cell-siNC-input

GSM3983230 mouse-active-T-cell-siH2A.Z-input

GSM3983231 mouse-active-T-cell-siNC-NS

GSM3983232 mouse-active-T-cell-siNC-Rnase-treated-NS

GSM3983233 mouse-active-T-cell-siH2A.Z-NS