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Extended Data Fig. 3 | Generation of the MFG (Mds1GFP/+ Flt3Cre) mice results in
restriction of GFP expression to LT-HSCs. a, Schematic of genetic strategy to
restrict GFP expression to LT-HSC compartment. b, Analysis of bone marrow
from Flt3CreR 2 6 L S L-To m mice shows Flt3Cre-driven activity in compartments
downstream of LT-HSCs. n = 4 mice; mean ± s.d. c, Further characterization
of the CKIT+SCA1−GFP+ cells from MFG mice. CD41+CD150+
cells represent megakaryocyte progenitors. The experiment was performed
twice with similar results. d, Flow characterization of MFG cell in marrow
isolated from multiple bones. The experiment was performed three times
with similar results. e, MFG-HSCs are predominantly found within the
CD34−Flt3−CD150+ bone marrow fraction. The experiment was performed
twice with similar results. f, MFG-HSCs are predominantly found within the
SCA1highEPCR+ bone marrow fraction. The experiment was performed once.
g, Cell cycle analysis of SLAM cells that are either GFP+ or GFP− in MFG mice.
Pooled data from three mice.