Nature - USA (2020-02-13)

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nature research | reporting summary

April 2018

Data

Policy information about availability of data

All manuscripts must include a data availability statement. This statement should provide the following information, where applicable:

• Accession codes, unique identifiers, or web links for publicly available datasets


• A list of figures that have associated raw data


• A description of any restrictions on data availability
  Source data for quantifications are available from the corresponding author upon request. There are no restrictions on data availability.

Field-specific reporting

Please select the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/authors/policies/ReportingSummary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size To determine the number of animals required to complete the proposed

studies, we performed power analyses based on literature review and data from our laboratory using the SPSS statistical software. Too high

numbers were avoided to keep the number of experimental animals as low as possible. All cell-based experiments had at least 3 biological

replicates.

Data exclusions No samples or animals were excluded.

Replication Experimental findings were successfully replicated, by either the same author(s) or a different author.

Randomization Age/sex matched litter mates were randomly assigned to groups. For magnetic force application experiments experiments, cells with 1-3

beads were randomly selected.

Blinding The investigators were blinded to group allocation/treatments during data collection. Most of the analysis was performed in an automated/

semi-automated fashion.

Reporting for specific materials, systems and methods

Materials & experimental systems

n/a Involved in the study

Unique biological materials

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Unique biological materials

Policy information about availability of materials

Obtaining unique materials Mouse cell lines, adenoviral constructs unique to this study can be obtained from the corresponding author upon reasonable

request.

Antibodies

Antibodies used phospho(p)-ERK1/2T-202;Y-204 (9106, Cell Signaling Technology, 1:1000), total(t)-ERK1/2 (9102, Cell Signaling Technology,