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Extended Data Fig. 1 | Characterization of KI cells stably expressing
f luorescent protein-labelled proteasome subunit or ubiquitin. a, HCT116
cells stably expressing proteasome subunits (PSMB2 or PSMD6) or ubiquitin
fused with either eGFP or FusionRed. Scale bars, 10 μm. b, Proteasome
assembly state of cell lines indicated in a, as determined by native PAGE.
c, d, Immunoblot analysis of the KI cells in a with antibodies against
ubiquitin (c), PSMD6 or PSMB2 (d). Ponceau S staining was performed as a
loading control. e, Cell viability of DBeQ-treated cells was determined using the
CellTiter assay kit. The MTS assay was performed in triplicate. Data are
mean ± s.d. (n = 3). In a–d, representative results from two independent
experiments. Gel source data for b–d are shown in Supplementary Fig. 1.