3
nature research | reporting summary
October 2018PSMD6, 1:1000, Enzo, BML-PW8225-0100, 01041704
PSMA1, 1:500, Kumatori A, Tanaka K et al. PNAS, 1990
VCP (5), 1:500, Abcam, ab11433, GR3180147-3
RAD23B (H-120), 1:500, Santa Cruz, sc-67225, A0809
RAD23B (D4W7F), 1:1000, Cell Signaling Technology, 13525, 1
RAD23A (D7U7Z), 1:1000, Cell Signaling Technology, 24555, 1
UBE3A, 1:100 (for IF) or 1:1000 (for WB), Protein Tech, 10344-1-AP, 00010503
RPL15, 1:500, Protein Tech, 16740-1-AP, 00008177
RPL7A (E109), 1:100, Cell Signaling Technology, 2415,2
RPL29 (B01P), 1:50, Abnova, H00006159-B01P), GB251
RPL35, 1:50, Sigma, SAB4500233, 3114173
RPL4 (RQ-7), 1:50, Santa Cruz, sc-100838, A3017
RPS2 (EPR10834(B)), 1:100, Abcam, ab155961, GR117602-1
RPS9, 1:100, Proteintech, 18215-1-AP, 00009939
RPS6 (5G10), 1:200, Cell Signaling Technology, 2217, 7
rRNA (Y10b), 1:100, Santa Cruz, sc-33678, A0510
PML (PG-M3), 1:100, Santa Cruz, sc-966, A0313
Coilin, 1:1000, Abcam, ab11822, GR127265-2
BMI1 (D20B7), 1:600, Cell Signaling Technology, 6964, 1
SC35, 1:100, Abcam, ab11826, GR144730-2
CENPC, 1:1000, MBL, PD030, 004
phospho-Histone H2A.X (Ser139) (JBW301), 1:100, Millipore, 05-636, 2310335
Cleaved Caspase-3 (Asp175) (5A1E), 1: 1000, Cell Signaling Technology, 9661, 42
FLAG (M2), 1:2000, Sigma, A8592, SLBH1183V
Actin, 1:4000, MBL, PM053-7, 007
Alexa Fluor 488–conjugated anti–mouse, 1:1000, Invitrogen, A-11029, 1252783
Alexa Fluor 488–conjugated anti–rabbit, 1:1000, Invitrogen, A-11034, 1073084
Alexa Fluor 568–conjugated anti–mouse, 1:1000, Invitrogen, A-11031, 1736975
Alexa Fluor 568–conjugated anti–rabbit, 1:1000, Invitrogen, A-11036, 1832035
Alexa Fluor 568–conjugated anti–guinea pig, 1:1000, Invitrogen, A-11075, 1170591
Alexa Fluor 647–conjugated anti–mouse, 1:1000, Invitrogen, A-21236, 1511347
Alexa Fluor 647–conjugated anti–rabbit, 1:1000, Invitrogen, A-21245, 1892148A
HRP-conjugated goat anti-mouse Ig, 1:10000, Jackson ImmunoResearch Laboratories, 115-035-003
HRP-conjugated goat anti-rabbit Ig, 1:10000, Jackson ImmunoResearch Laboratories, 111-035-144, 55285Validation Ubiquitin (Dako, Z0458): Previously used for publication (Tsuchiya H., Mol Cell. 2017 May 18;66(4):488-502.e7.), and (Kageyama
M., PLoS One. 2019 May 31;14(5):e0217945.).
Ubiquitin (FK2, Nippon Bio-Test Laboratories, 0918-2): Previously used for publication (Nozawa T et al., Autophagy.
2017;13(11):1841-1854.).
Ubiquitin, Lys48-Specific (Millipore, 05-1307): Supplier has been validated in Flow cytometry analysis of Jurkat cells,
Immunocytochemistry of NIH/3T3, HeLa, and A431 cells, Immunoprecipitation via precipitated ubiquitin-containing proteins
from HeLa cell lysates, Immunohistochemistry of rat brain sections and human breast carcinoma, and Western blotting of the
inhibition assay using HeLa cell lysate.
Ubiquitin, Lys63-Specific (Millipore, 05-1308): Supplier has been validated in Flow cytometry analysis of Jurkat cells,
Immunocytochemistry of staining of colorectal cancer tissue sections limited to tumor cells, Immunoprecipitation via
precipitated ubiquitin-containing proteins from HeLa cell lysates, Immunohistochemistry of staining of colorectal cancer tissue
sections limited to tumor cells, and Western blotting of the inhibition assay using HeLa cell lysate.
PSMB2 (Enzo, BML-PW8890-0100): Previously used for publication (Zana Lukic et al., Retrovirology. 2011; 8: 93.)
PSMD6 (Enzo, BML-PW8225-0100): Previously used for publication (Mata-Cantero L et al., J Proteomics. 2016 Apr 29;139:45-59.)
PSMA1: Previously used for publication (Kumatori A, Tanaka K et al., Proc Natl Acad Sci U S A. 1990 Sep;87(18):7071-5.)
VCP (Abcam, ab11433): Supplier has been validated in Western blotting of cell lysate from mouse embryonic fibroblasts and
CA46 cells, Immunohistochemistry of Human colon carcinoma tissues limited to tumor cells, Flow cytometry analysis of Platelets,
and Immunocytochemistry of WiDr colon carcinoma cells.
RAD23B (Santa Cruz, sc-67225): Supplier has been validated in Immunohistochemistry of human cerebellum, and
Immunocytochemistry of human cell line U-2 OS cells.
RAD23B (Cell Signaling Technology, 13525): Supplier has been validated in Western blotting of extracts from 293T cells.
RAD23A (Cell Signaling Technology, 24555): Supplier has been validated in Western blotting of extracts from SK-OV-3, OVCAR3,
CCRF-CEM, RPMI 8226, 786-O, A498, Hepa 1-6, M-1, C6, A-10, COS-7 and 293T cells.
UBE3A (Protein Tech, 10344-1-AP): Supplier has been validated in Western blotting of mouse brain tissue or extracts from
HEK293, HeLa, Jurkat and K562 cells, Immunohistochemistry of paraffin-embedded human lung cancer tissue, and
Immunocytochemistry of HeLa cells.
RPL15 (Protein Tech, 16740-1-AP): Supplier has been validated in Western blotting of COLO 320 cells, and Immunocytochemistry
of HeLa cells.
RPL7a (Cell Signaling Technology, 2415): Supplier has been validated in Western blotting of extracts from HEK293, NIH/3T3, PC12
and COS cells, and Immnocytochemistry of COS cells.
RPL29 (Abnova, H00006159-B01P): Supplier has been validated in Western blotting of extracts from human pancreas,
Immunocytochemistry of HeLa cells.
RPL35 (Sigma, SAB4500233): Supplier has been validated in Immunoblotting analysis of extracts from 293, HeLa, COLO and
Jurkat cells.
RPL4 (Santa Cruz, sc-100838): Supplier has been validated in Immunoblotting analysis of extracts from HeLa, Jurkat and K-562
cells, and Immunocytochemistry of HeLa cells.
RPS2 (Abcam, ab155961): Supplier has been validated in Immunohistochemistry of Human kidney and pancreas tissue, Western
blotting of the inhibition assay using HeLa, A549 and 293T cell lysate, and Immunocytochemistry of HeLa cells.
RPS9 (Proteintech, 18215-1-AP): Supplier has been validated in Western blotting of extracts from mouse uterus rissue,
Immunocytochemistry of Human kidney, and Immunocytochemistry of HeLa cells.