Nature - USA (2020-02-13)

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nature research | reporting summary

April 2018

Data

Policy information about availability of data

All manuscripts must include a data availability statement. This statement should provide the following information, where applicable:

• Accession codes, unique identifiers, or web links for publicly available datasets


• A list of figures that have associated raw data


• A description of any restrictions on data availability
  Data that support the findings of this study are available from the corresponding author upon reasonable request.

Field-specific reporting

Please select the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/authors/policies/ReportingSummary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size Sample size for yeast experiments was determined by referring to commonly used size.

Data exclusions No exclusion.

Replication Quantitative FRAP experiments were repeated three times with consistent data. FCS analyses were performed for dozens of cells in order to

obtain statistically reliable data. Observation of coalescence of droplets was repeated three times both in vivo and in vitro with consistent

data. Observation of Ostwald ripening was repeated three times with consistent data. Phosphorylation and dephosphorylation experiments in

vitro were repeated three times with consistent data. HS-AFM observation of scaffold droplets were repeated three times with consistent

data. GUV experiments were repeated at least five times with consistent data. Observation of PAS localization in yeast cells was repeated

three times with consistent data.

Randomization Randomization is not relevant for this study because our work does not involve clinical trials or population studies.

Blinding Blinding is not relevant for this study because no group allocation was performed.

Reporting for specific materials, systems and methods

Materials & experimental systems

n/a Involved in the study

Unique biological materials

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used Anti-FLAG M2 (F3165; Sigma; Lot#SLBN8915V)(dilution 1:1000), Anti-HA TANA2 (M180-3S; MBL; Lot#003)(dilution 1:10000),

Anti-Mouse IgG (Fab specific)–Peroxidase antibody, polyclonal (A9917; Sigma; Lot#081M4762)(dilution 1:20000), Anti-Rabbit IgG

(whole molecule)–Peroxidase antibody, polyclonal (A6154; Sigma; Lot# SLBK2462V)(dilution 1:20000), Anti-Atg1-T226-P

(produced in this study)(dilution 1:2000), Anti-Atg13-S428/9-P (Nat Struct Mol Biol 21, 513, 2014)(dilution 1:2000)

Validation Validation for anti-FLAG M2 was performed by detecting a single band of protein on a western blot from an E. coli crude

cell lysate: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/f3165dat.pdf; Validation for anti-

HA was performed by detecting a band of N-terminal HA fusion proteins in cell extracts: https://www.sigmaaldrich.com/content/

dam/sigma-aldrich/docs/Sigma/Datasheet/2/h3663dat.pdf; anti-mouse IgG: https://www.sigmaaldrich.com/content/dam/