Nature - USA (2020-02-13)

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nature research | reporting summary

April 2018

Data

Policy information about availability of data

All manuscripts must include a data availability statement. This statement should provide the following information, where applicable:

• Accession codes, unique identifiers, or web links for publicly available datasets


• A list of figures that have associated raw data


• A description of any restrictions on data availability
  RNASeq and ChIPSeq dataset can be accessed in GEO (Accession GSE118152, GSE118247, GSE130269). Crystal coordinates and X-ray diffraction data was deposited
  in the protein databank with the accession code 6E6J and 6ONY. Other datasets generated and/or analyzed during the current study are available from the
  corresponding author on reasonable request.

Field-specific reporting

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Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

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Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size For efficacy studies, a one sided t-test was used to determine the number of

animals needed to obtain 80% power at alpha = 0.05. For rat tox studies, sample size of n=3 animals per group was based on internal

experience of ability to identify test article related changes during drug candidate selection.

Data exclusions No data were excluded from the analysis.

Replication Experiments were repeated with same conditions and obtained similar results. The number of repeats were indicated in figure legends.

Randomization For efficacy study, mice were randomized into treatment groups using Studylog software (Studylog Systems, Inc., South San Francisco, CA)

based on tumor volume. For rat tox study, animal allocation to vehicle and treatment groups was at random based on body weight.

Blinding Partial blinding for efficacy studies was used. A multiple technicians formulated and dosed compounds and randomized the groups. Additional

investigators blinded to the test agents measured tumor volumes during the study. Toxicologic data analysis is generally performed in

unblinded fashion which was the case for data described in this paper.

Reporting for specific materials, systems and methods

Materials & experimental systems

n/a Involved in the study

Unique biological materials

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used Information on all of the antibodies used in the study is presented in SI Table

Validation H3K27Ac Ab noted on Active motif website to be modENCODE validated, NGS-QC certified, and validated for ChIP-Seq. BRD4 Ab

is cited in at least 11 literature publications for ChIP and ChIP-Seq. AR Ab is cited in at least 28 literature publications including

ChIP and ChIP-Seq applications. Antibody information is presented in SI Table.