Extended Data Fig. 2 | Pf HT1 has evolved to be an eff icient polyspecif ic sugar
transporter. a, Size-exclusion chromatogram of DDM-purified Pf HT1 showing
Pf HT1 migrates as two oligomeric species (dimer and monomer); the sample
migrates as a monomer during SDS–PAGE. b, Time-dependent uptake of [^14 C]
d-mannose (black circles) by Pf HT1 in proteoliposomes. Inset, Pf HT1 uptake of
radiolabelled sugar (cyan trace) compared with non-specific uptake estimated
from radioactivity measured from liposomes incubated without protein (black
trace). Error bars represent the mean ± s.e.m. of n = 3 biologically independent
experiments. c, As in b, for [^14 C]d-galactose. d, As in b, for [^14 C]d-fructose. e, As
in b, for [^3 H]d-x ylose. f, As in b, for [^3 H]d-glucosamine.