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NEBNext

®

Single Cell/Low

Input RNA Library Prep Kit

With this unique streamlined method, high-

quality, full-length transcript sequencing libraries

are made directly from single cells, or from as

little as 2 pg – 200 ng of total RNA.

• Detect more transcripts, including

low-abundance transcripts

• Obtain uniform, full-length transcript coverage,

regardless of input amount or sample type

• Save time with a streamlined workflow,

minimal handling steps and hands-on time

How low

can you go?

One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, For more information, please email us at [email protected]. Inc.

The use of these products may require you to obtain additional third party intellectual property rights for certain applicationILLUMINA®, NEXT-SEQ® and NEXTERA® are registered trademarks of Illumina, Inc. SMART-SEQ® and ULTRA® are registered trademarks of Takara Bio, Incs.

© Copyright 2018, New England Biolabs, Inc.; all rights reserved.

Superior transcript detection with the NEBNext

Single Cell/Low Input RNA Library Prep Kit

Sequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext

Single Cell/Low Input RNA Library Prep Kit, or the SMART-Seq® v4 Ultra® Low Input RNA

Kit for Sequencing plus the Nextera® XT DNA Library Prep Kit. Libraries were sequenced

on an Illumina® NextSeq® 500. Each dot represents the number of transcripts identified at the

given Transcripts Per Kilobase Million (TPM) range, and each box represents the median,

first and third quartiles per replicate and method. Salmon 0.6 was used for read mapping and

quantification of all GENCODE v25 transcripts. Increased identification of low abundance

transcripts is observed with the NEBNext libraries.

Visit NEBNext.com

to request your sample today.

1: NEBNext® Kit 2: SMART-Seq® v4/Nextera® XT

Number of Transcripts

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>50 TPM

Full Stack

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CRISPR Applications for Engineered Cells