The_20Scientist_20March_202019 (1)

(singke) #1
be INSPIRED
driveDISCOVERY
stay GENUINE

NEBNext


®
Single Cell/Low

Input RNA Library Prep Kit


With this unique streamlined method, high-


quality, full-length transcript sequencing libraries


are made directly from single cells, or from as


little as 2 pg – 200 ng of total RNA.



  • Detect more transcripts, including


low-abundance transcripts



  • Obtain uniform, full-length transcript coverage,


regardless of input amount or sample type



  • Save time with a streamlined workflow,


minimal handling steps and hands-on time


How low


can you go?


OneFor more information, or more of these products please email are covered us at [email protected]. by patents, trademarks a n d /o r copyrights owned or controlled by New England Biolabs, Inc.
TheILLUMINA use of these®, NEXT-SEQ products® may and require NEXTERA you to obtain® are registered additional trademarks third party of Illumina, intellectual Inc. property SMART-SEQ rights® for and certain applications. ULTRA® are registered trademarks of Takara Bio, Inc
© Copyright 2018, New England Biolabs, Inc.; all rights reserved.

Superior transcript detection with the NEBNext
Single Cell/Low Input RNA Library Prep Kit

Sequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext
Single Cell/Low Input RNA Library Prep Kit, or the S M A RT- S e q® v4 Ultra® Low Input RNA
Kit for Sequencing plus the Nextera® XT DNA Library Prep Kit. Libraries were sequenced
on an Illumina® NextSeq® 500. Each dot represents the number of transcripts identified at the
given Tr a n s c r i p t s Per Kilobase Million (TPM) range, and each box represents the median,
first and third quartiles per replicate and method. Salmon 0.6 was used for read mapping and
quantification of all GENCODE v25 transcripts. Increased identification of low abundance
transcripts is observed with the NEBNext libraries.

Visit NEBNext.com
to request your sample today.

1: NEBNext® Kit 2: SMART-Seq® v4/Nextera® XT

Number of Transcripts

0

500

1000

1500

2000

2500

3000

3500

4000

4500

1 2

1–5 TPM

0

500

1000

1500

2000

2500

3000

1 2

5–10 TPM

0

1000

2000

3000

4000

5000

6000

7000

1 2

10–50 TPM

0

500

1000

1500

2000

2500

3000

3500

1 2

>50 TPM
Free download pdf