be INSPIRED
driveDISCOVERY
stay GENUINENEBNext
®
Single Cell/LowInput RNA Library Prep Kit
With this unique streamlined method, high-
quality, full-length transcript sequencing libraries
are made directly from single cells, or from as
little as 2 pg – 200 ng of total RNA.
- Detect more transcripts, including
low-abundance transcripts
- Obtain uniform, full-length transcript coverage,
regardless of input amount or sample type
- Save time with a streamlined workflow,
minimal handling steps and hands-on time
How low
can you go?
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© Copyright 2018, New England Biolabs, Inc.; all rights reserved.Superior transcript detection with the NEBNext
Single Cell/Low Input RNA Library Prep KitSequencing libraries were generated from Jurkat single cells (6 replicates) using the NEBNext
Single Cell/Low Input RNA Library Prep Kit, or the S M A RT- S e q® v4 Ultra® Low Input RNA
Kit for Sequencing plus the Nextera® XT DNA Library Prep Kit. Libraries were sequenced
on an Illumina® NextSeq® 500. Each dot represents the number of transcripts identified at the
given Tr a n s c r i p t s Per Kilobase Million (TPM) range, and each box represents the median,
first and third quartiles per replicate and method. Salmon 0.6 was used for read mapping and
quantification of all GENCODE v25 transcripts. Increased identification of low abundance
transcripts is observed with the NEBNext libraries.Visit NEBNext.com
to request your sample today.1: NEBNext® Kit 2: SMART-Seq® v4/Nextera® XTNumber of Transcripts0500100015002000250030003500400045001 21–5 TPM0500100015002000250030001 25–10 TPM010002000300040005000600070001 210–50 TPM05001000150020002500300035001 2>50 TPM