(^46) (^) Application Note
The N-glycans of antibody Fc domains are known to play an
important role in Fc-mediated effector functions. Accordingly,
separation patterns of therapeutic antibodies on TSKgel FcR-
IIIA-NPR can be correlated to mAb glycoforms. Early eluting
peaks (low affinity to Fc receptor) represent mAb glycoforms
with low ADCC activity while late eluting peaks represent
glycoforms with high ADCC activity.
Chromatographic conditions:
Column: TSKgel FcR-IIIA-NPR (5 μm 4.6 x 75 mm); Mobile
Phase: A: 50 mM Citrate, pH 6.5; B: 50mM Citrate, pH 4.5;
Flow rate: 1 mL/min; Detection: UV@280 nm; Sample: Rituximab
Read the full application note at http://bit.ly/FcR-2p
Evaluation of mAb
ADCC Activity through
Fc Receptor Affinity
Chromatography
Antibody-dependent cell-mediated
cytotoxicity (ADCC) is a mechanism of action
of anti-tumor antibodies in which the Fcy
receptor (FcR) plays a key role. Hence, affinity
chromatography using recombinant FcR can
deliver valuable information about ADCC
activity. Data on the analysis of therapeutic
mAbs with the new TSKgel FcR-IIIA-NPR HPLC
column are provided.
FASTER AND LESS EXPENSIVE THAN CURRENT ADCC ACTIVITY ASSAYS. CHECK OUT THE NEW SOLUTION
FOR ANTIBODY ADCC ACTIVITY SCREENING AND APPLY FOR A TEST COLUMN AT bit.ly/FcR-2p
FAST CELL LINE SCREENING, UPSTREAM DEVELOPMENT, LOT-TO-LOT COMPARISON
EASY AND REPRODUCIBLE HPLC ANALYSIS BASED ON FC γ RECEPTOR AFFINITY OF mAbs
PERFORM OVER 40 RUNS PER DAY AND HUNDREDS OF RUNS PER COLUMN
A NEW TOOL FOR QUICK ADCC ACTIVITY ASSESSMENT
FcR-IIIA affinity chromatography separates mAb glycoforms according to
their ADCC activity
singke
(singke)
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