Application Note ^47
FASTER AND LESS EXPENSIVE THAN CURRENT ADCC ACTIVITY ASSAYS. CHECK OUT THE NEW SOLUTION
FOR ANTIBODY ADCC ACTIVITY SCREENING AND APPLY FOR A TEST COLUMN AT bit.ly/FcR-2p
FAST CELL LINE SCREENING, UPSTREAM DEVELOPMENT, LOT-TO-LOT COMPARISON
EASY AND REPRODUCIBLE HPLC ANALYSIS BASED ON FC γ RECEPTOR AFFINITY OF mAbs
PERFORM OVER 40 RUNS PER DAY AND HUNDREDS OF RUNS PER COLUMNA NEW TOOL FOR QUICK ADCC ACTIVITY ASSESSMENT
Solvent is delivered to the open port sampling area through the outer
pair of concentric tubes at ~250 μL/ min. Paired with the electrospray
ion source of the expression Compact Mass Spectrometer, the solvent
forms a meniscus at the open port before being drawn down the inner
path into the ion source of the mass spectrometer under the Venturi
effect of the nebulization gas. The control and fentanyl-spiked urine
samples, contained in a pipette tip, are simply touched in sequence to
the OPSI sampling port and results are obtained in < 30 sec.
The mass spectra of fentanyl and fentanyl-D5 (Figure 1) show
protonated molecules at m/z 337.2 for fentanyl and 342.2 forfentanyl-D5. These ions are used in a Selected Ion Monitoring
(SIM) method for the quantification of fentanyl in urine. The
OPSI sample technique provides an effective, high-throughput
assay for fentanyl screening applications.For more information visit http://www.advion.comTouch Express OPSI
coupled to the Advion
expression CMS provides
a simple, fast, direct
sampling method for
fentanyl screening in urine
Touch ExpressTM Open Port Sampling Interface (OPSI) for direct analysis of
solids, liquids, surfaces and fi bers with the expression Compact Mass Spectrometer
The OPSI is a one-touch sampling technique
that provides mass analysis in seconds - no
preparation or chromatography required- Compound identifi cation and impurity detection
from almost any liquid or surface - Easy screening applications for drug
research, food safety, environmental, and
forensics - Large molecule applications, including
proteins, peptides, oligonucleotides and
polymers
Advion.com/OPSI • [email protected]/OPSI • [email protected]/OPSI • [email protected]
“Strokes of Genius”Figure 1: Mass spectra of Fentanyl & Fentanyl-D5 in control urine.Touch ExpressTM Open Port Sampling Interface (OPSI) for direct analysis of
solids, liquids, surfaces and fi bers with the expression Compact Mass Spectrometer
The OPSI is a one-touch sampling technique
that provides mass analysis in seconds - no
preparation or chromatography required- Compound identifi cation and impurity detection
from almost any liquid or surface - Easy screening applications for drug
research, food safety, environmental, and
forensics - Large molecule applications, including
proteins, peptides, oligonucleotides and
polymers
Advion.com/OPSI • [email protected]/OPSI • [email protected]/OPSI • [email protected]
“Strokes of Genius”