Sjöstedtet al.,Science 367 , eaay5947 (2020) 6 March 2020 10 of 16
ENPP6OligodendrocyteSLC13A5AstrocyteALDH1L1ALOX5MicrogliaPYCARD SNTA1ITGB2NeuronKCNAB2KCNB1PDE1BPPP1R14AMAGACDLow tissuespecificityLymphoidtissuesBloodBone marrowLiverMuscle tissueAdiposeSkinRetinaLungMouthtissuesFemaleGI tract
Kidney
Endocrine
glands
Pituit
Pancreasary gland
Male
tissuesBladderBrainBAstrocy
teMric
go
ialOligode
ndro
Neuron cyteBrainGastrointestinal tractKidneyEndocrine glandsPituitary glandPancreasBladderMale tissuesFemale tissuesMouthLungRetinaSkinAdiposeMuscle tissuesLiverBone marrowBloodLymphoid tissuesNormalized expression (NX)AstrocyteMicrogliaNeuron
OligodendrocyteACACBC1QB PLTPCCDC3GREM2FBLN1CD37 FCGR3B
CD14FXYD5
HCST
ITGB2SLC17A7
ATP1A3APOEAPOENOV
CD109CCDC80 FBLN1 EBI3CDH1
FOLH1
MYO5B
CALB1
ALDH4A1 APOECD14 SDS APOEC1QB
C1QAHLA−DMAC1QC C1QB
C1QACD38FBLN1
CCDC80CLCA4
PYGM
KIAA1324PYGM
S100A1
MYBPC1CDH1
PLTPKIAA1324RIMBP2TUSC3
KIAA1324PLA2G5 RLBP1RGRFGFR3
RAPGEFL1HES1200 400 600 800012Microglia Astrocyte Neuron OligodendrocyteRegional coefficient of variation (CV)RLBP1FBLN1MFNG ALOX5SUCNR1 RGS20 NWD1 LGR5 ENPP6SLC5A11HOXD1
FREM2 YSFP2RY1 PDE1B NDST3 IP6K3ST8SIA5 GAD2TBR1 TRHDE
GRIN2C PLCB4GRM1RNF43 HTR2C
ANO3 RELNDRD1ZIC5PCSK1
DKidneySmooth muscleSkeletal muscleLiverSpleen LiverSpleenSpleenFig. 7. The gene expression landscape of 420 putative brain cell type signature
genes in cerebral cortex.(A) The expression variation for the 420 signature
genes in the different brain regions was analyzed on the basis of the coefficient
of variation (CV) of NX values across the 10 regions (see figs. S33 and S34
for expression heatmap and scatterplot across the 10 regions). (B)Theexpression
levels for the individual signature genesin the different tissue types. The most
abundant genes are indicated by gene names (see fig. S32 for expression
heatmap of the signature genes across all tissues). (C) Chord diagram shows
the tissue specificity of brain cell type signature genes. Each link represents the
number of cell type signature genes that are elevated at a tissue level in the
analyzed organ and tissue types. (D) Examples of signature genes and their
protein localization in the different cell types. Microglia signature genes
(ALOX5, ITGB2, and PYCARD) are highly expressed in spleen. Several astrocyte
signature genes, including SLC13A5 and ALDH1L1, have elevated expression in
liver, whereas SNTA1 is highly expressed in skeletal muscle. Neuronal examples
(KCNAB2, KCNB1, and PDE1B) are located in neuronal cell bodies in the brain,
while oligodendrocyte proteins, including MAG, are detected in white matter of
the brain as well as in smooth muscle (PPP1R14A) and renal tubule of kidney
(ENPP6). More detailed expression profiles for all cell signature genes are shown
in figs. S33 to S35. Scale bars, 25mm.RESEARCH | RESEARCH ARTICLE