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ACKNOWLEDGMENTS
We thank P. Aebischer, P. Hegemann, A. Y. Karpova, R. McQuiston,
D. V. Schaffer, N. Shah, J. Wells, and J. Wietek for sharing their
plasmids and H. Hioki for providing anti-VGLUTs antibodies.
Funding:This study was supported by the JSPS NEXT program
(LS070 to Ka.N.); MEXT KAKENHI (16H05128, 15H05932,26118508, 26713009, and 22689007 to Ka.N.; 19K06954 and
16K19006 to N.K.); JST PRESTO (JPMJPR13M9 to Ka.N.); AMED
(JP19gm5010002 to Ka.N.); and grants from the Takeda Science
Foundation, Nakajima Foundation, Uehara Memorial Foundation,
Ono Medical Research Foundation, Brain Science Foundation,
Kowa Life Science Foundation (to Ka.N.), and Kato Memorial
Bioscience Foundation (to N.K.).Author contributions:Ka.N.
conceived the project. N.K. and Ka.N. designed experiments. All
authors performed experiments and analyzed data. N.K. and
Ka.N. discussed data and wrote the manuscript. Ka.N. supervised
the entire project. All authors approved the manuscript.
Competing interests:The authors declare no competing interests.
Data and materials availability:All data are available in the
main text or the supplementary materials. Plasmids and AAVs with
Addgene numbers given in the materials and methods were
obtained under material transfer agreements with Addgene.SUPPLEMENTARY MATERIALS
science.sciencemag.org/content/367/6482/1105/suppl/DC1
Materials and Methods
Figs. S1 to S8
Table S1
References ( 33 – 38 )
View/request a protocol for this paper fromBio-protocol.12 September 2019; accepted 4 February 2020
10.1126/science.aaz4639Kataokaet al.,Science 367 , 1105–1112 (2020) 6 March 2020 8of8
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