Science - USA (2020-02-07)

DELLAs in a loss-of-functionslr1mutant (Fig. 3E) ( 11 ) or in the presence of the high-level DELLA accumulation conferred by the ...

(fig. S11C). Thus, in addition to both being sub- strates of gibberellin-GID1-SCFGID2, SLR1 and NGR5 interact directly with one ...

9311-sd1. Thus, the enhanced DELLA function ofsd1increases tiller number in response to nitrogen supply by increasing the stabil ...

manufacturer’s user guide. cDNAs encod- ing various deleted and nondeleted versions of SLR1 were amplified and then subcloned in ...

and terminated by adding SDS sample buffer withb-mercaptoethanol. Reaction products were separated with 12% SDS-PAGE and sub- je ...

S. Wanget al., Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant t ...

RESEARCH ARTICLE SUMMARY ◥ IMMUNOLOGY Butyrophilin 2A1 is essential for phosphoantigen reactivity bygdT cells Marc Rigau, Simone ...

RESEARCH ARTICLE ◥ IMMUNOLOGY Butyrophilin 2A1 is essential for phosphoantigen reactivity bygdT cells Marc Rigau1,2,3, Simone Os ...

andBTN2A1null2), with similar results also from a distinct LM-MEL-75BTN2A1-mutant cell line (Fig. 1C and fig. S3). This was in- ...

Rigauet al.,Science 367 , eaay5516 (2020) 7 February 2020 3of13 A C BTN2A1 tetramer SAv. control BTN2A1 tetramer 2 0.5 2 0.1 0.1 ...

for classicalabT cells ( 23 ). It also bound a “hybrid”gdTCR that coexpressed the TCR 6gchain paired with an irrelevant Vd 1 +d ...

Rigauet al.,Science 367 , eaay5516 (2020) 7 February 2020 5of13 C F D E V δ^2 +γδ T cells (x10 -4 ) WT 2A1null1 0 1 2 3 Unstimul ...

association (fig. S14). Thus, both the extra- cellular and intracellular domains of BTN2A1 and BTN3A1 are closely associated. Vg ...

pAg-mediated activation (Fig. 6C) ( 10 ). These residues had no effect on BTN2A1 binding (Fig. 6B) and were located on the oppos ...

(dchain) and Lys^108 (gchain), all failed to respond to parental APCs pulsed with agonist anti-BTN3A1 mAb (Fig. 7). Thus, an int ...

it unlikely that CD209 plays a role in this in- teraction. Although little is known about the expression pattern of BTN2A1, RNA ...

RPMI-1640 and AIM-V (Invitrogen) supple- mented with 10% (v/v) FCS (JRH Biosciences), penicillin (100 U/ml), streptomycin (100mg ...

with streptavidin-PE (BD) at a 4:1 molar ratio. DNA constructs encoding butyrophilin B30.2 intracellular domains with C-terminal ...

BTN2A1 or BTN3A1 B30.2 domains were buf- fer exchanged into PBS and adjusted to a final concentration of 100mM. HMBPP (Cayman Ch ...

S. Chenet al., Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.Cell 160 , 1246–1260 (2015). doi:10.10 ...