Science - USA (2020-02-07)

RESEARCH ARTICLE ◥ SIGNAL TRANSDUCTION Mechanism of homodimeric cytokine receptor activation and dysregulation by oncogenic muta ...

(Fig. 1B). Taking into account an effective labeling degree of ~70% achieved by both Rho11NB andDY647NB (fig. S4C), the total ce ...

Spatial cross-correlation above the background was observed only after adding the ligand (Fig. 1F, fig. S6, C and D, and table S ...

Single-molecule photobleaching confirmed that JAK2 V617F induced formation of TpoR dimers, and single-molecule FRET (smFRET) con ...

signaling complex, we converted dimerization levels into two-dimensional equilibrium disso- ciation constants (KD2D) according t ...

Wilmeset al.,Science 367 , 643–652 (2020) 7 February 2020 6of10 B E F pJAK2 V617F JAK2 pSTAT5 0.00 0.05 0.10 0.15 0.20 0.25 0.30 ...

can shift the equilibrium toward receptor dimers, as observed for TpoR W515L and JAK2 V617F. Weak intrinsic receptor dimerizatio ...

in the context of receptor-JAK2 monomers (group III), and (ii) stabilization of receptor- JAK2 dimers (groups I and II). Similar ...

of hydrophobic and polar interactions at the interface (tables S5 and S6). Interactions of certain key residues, such as Glu^592 ...

A. V. Tomset al.,Nat.Struct.Mol.Biol. 20 , 1221– 1223 (2013). R. M. Bandaranayakeet al.,Nat. Struct. Mol. Biol. 19 , 754– 759 ( ...

SIGNAL TRANSDUCTION An AMPK–caspase-6 axis controls liver damage in nonalcoholic steatohepatitis Peng Zhao^1 †, Xiaoli Sun^1 †, ...

cytokine, which is consistent with similar macro- phage infiltration in Flox and LAKO mice. Like- wise,LAKOmiceshowednodifferenc ...

LAKO had no effect on body weight, liver weight, liver-to–body weight ratio, hepatic TG, fasting glucose, glucose tolerance, or ...

further exaggerate steatosis or inflammation during NASH, hepatocellular death and fibro- sis were enhanced. AMPK deficiency inc ...

active caspase-6 staining, the degree of active caspase-6 was increased in cirrhosis (Fig. 3D). Moreover, whole-section scanning ...

for 6 weeks to establish NASH and then in- traperitoneally injected mice with vehicle or AMPK agonist (A-769662) for 2 weeks whi ...

liver damage (fig. S7, F to H). A-769662 atte- nuated hepatic fibrosis (Fig. 4, F to H) and sig- nificantly reduced the expressi ...

palmitic acid (PA) to mimic inflammation and lipotoxicity-induced hepatocellular death. Both induced caspase-6 activation (fig. ...

together, our data support the conclusion that caspase-6 is activated and participates in the pathogenesis of liver damage in NA ...

REPORT ◥ ELECTROCHEMISTRY CO 2 electrolysis to multicarbon products at activities greater than 1 A cm − 2 F. Pelayo García de Ar ...