Saunderset al.,Science 366 , eaay7199 (2019) 6 December 2019 8of17
Fig. 4. Vaccine-induced antibody DH270.mu1 with improbable mutations
binds to the HIV-1 Env SOSIP trimer with the same binding mode as
DH270 bnAbs.(A) Cryo-EM reconstruction (4.3 Å resolution) of DH270.6
(cyan) bound to CH848 10.17DT–stabilized Env trimers (gray) segmented by
components. (B) Same as (A) but for the DH270.mu1 complex (3.5 Å resolution)
with DH270.mu1 shown in pink. (C) Zoomed-in view of the interactive region
between DH270.6 and CH848 10.17DT. HIV-1 Env gp120 is shown in gray with the
V3 loop in orange and the V1 loop in red. Glycans are shown in stick repre-
sentation and colored by elements, with oxygen atoms in red and nitrogen atoms
in blue. The heavy chain of DH270.6 is shown in cyan and light chain
in yellow. Color code for CDRs: wheat, CDR H1; blue, CDR H2; purple, CDR H3;
brown, CDR L1; pink, CDR L2; teal, CDR L3. (D) Same as (C) but for the
DH270.mu1 complex, with DH270.mu1 heavy chain in pink. (E) Overlay of the
DH270-bound V1 (green), DH270.6-bound V1 (blue), and DH270.mu1-bound
V1 (magenta). (FtoH) Zoomed-in views showing details of the contacts
made by heavy chain residue 57. Black dashed lines indicate hydrogen bonds.
(ItoK) Zoomed-in views showing details of the contacts made by light
chain residue 27.RESEARCH | RESEARCH ARTICLE
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