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ACKNOWLEDGMENTS

We thank D. Vogel for assistance with the preparation of cultured

neurons; M. Heumüller and J. J. Letzkus for assistance with the

intracerebroventricular injections; I. Wüllenweber and F. Rupprecht

for assistance with the proteomics analysis; N. T. Ingolia and

M. J. McGlincy (Department of Molecular and Cellular Biology,

University of California, Berkeley) for advice on bioinformatic

analysis of footprint libraries; and E. Valjent (IGF, CNRS, INSERM,

University of Montpellier) for providing the Wfs1Cre transgenic

mice.Funding:A.B. is supported by an EMBO long-term

postdoctoral fellowship (EMBO ALTF 331-2017). E.M.S. is funded

by the Max Planck Society, an Advanced Investigator award

from the European Research Council (grant 743216), DFG CRC

1080: Molecular and Cellular Mechanisms of Neural Homeostasis,

and DFG CRC 902: Molecular Principles of RNA-based Regulation.

Author contributions:A.B. and C.G. designed and conducted

experiments and analyzed results. G.T. analyzed results. E.C. and

T.D. conducted experiments. J.D.L. acquired the proteomics

data. E.M.S. designed experiments and supervised the project. A.B.

and E.M.S. wrote the manuscript, and all authors edited the

manuscript.Competing interests:The authors declare no

competing interests.Data and material availability:All data are

available in the main text or the supplementary materials. The

accession number for the raw sequencing data reported in

this paper is NCBI BioProject: PRJNA550323. The mass

spectrometry proteomics data are deposited at the ProteomeXchange

Consortium via PRIDE ( 102 ) partner repository with the dataset

identifier PXD016552. All bioinformatic tools used in this study are

contained in one modular C++ program called RiboTools. The

source code and further notes on the algorithms can be found on

our GitHub repository ( 103 ). Other analysis scripts and codes

are available upon request.

SUPPLEMENTARY MATERIALS

science.sciencemag.org/content/367/6477/eaay4991/suppl/DC1

Figs. S1 to S15

Table S1

View/request a protocol for this paper fromBio-protocol.

24 June 2019; resubmitted 29 October 2019

Accepted 18 December 2019

10.1126/science.aay4991

Bieveret al.,Science 367 , eaay4991 (2020) 31 January 2020 14 of 14

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