Science - 06.12.2019

(fcrit< 4%). Geological observations and mod- els have suggested thatfcritmay range from <10% to >90% ( 18 , 19 ), but ...

have involved failure of deeper parts of the summit magma system. Globally,lavalakesarerare.Wheretheydo exist, close observation ...

E. P. Holohan, M. P. J. Schöpfer, J. J. Walsh, Mechanical and geometric controls on the structural evolution of pit crater and ...

RESEARCH ARTICLE SUMMARY ◥ HIV VACCINES Targeted selection of HIV-specific antibody mutations by engineering B cell maturation K ...

RESEARCH ARTICLE ◥ HIV VACCINES Targeted selection of HIV-specific antibody mutations by engineering B cell maturation Kevin O. ...

8ANC131) bnAb class because they all encode K19Tchangesaswell. Here, we designed immunogens that select for specific functional ...

Saunderset al.,Science 366 , eaay7199 (2019) 6 December 2019 3of17 Fig. 1. Structural characterization and development of an HIV ...

control mechanisms may also curtail DH270 precursorexpressioninhumans,thisDH270 VH+VLheterozygous mouse provides a relevant and ...

Saunderset al.,Science 366 , eaay7199 (2019) 6 December 2019 5of17 Fig. 2. CH848 10.17DT nanoparticle immunization of DH270 UCA ...

Saunderset al.,Science 366 , eaay7199 (2019) 6 December 2019 6of17 Fig. 3. CH848 10.17DT Env trimer nanoparticle immunization el ...

boosting immunogens designed to specifically select for combinations of key improbable mutations will be necessary to accelerate ...

Saunderset al.,Science 366 , eaay7199 (2019) 6 December 2019 8of17 Fig. 4. Vaccine-induced antibody DH270.mu1 with improbable mu ...

4 mM affinity (fig. S25A). The highest-affinity immunogen elicited the highest frequency of K19T-encoding antibodies, and the lo ...

present in CH235 (figs. S25 and S26). These three vaccine-induced antibodies more po- tently neutralized Man 5 GlcNAc 2 -enriche ...

must be precise, and the simultaneous use of sequential immunogens for multiple bnAb typesalsorunstheriskofleadingsomebnAb B cel ...

maturation is needed for a protective anti- body response. Methods Animals and immunizations DH270 UCA VHand VLKI mice were gen- ...

formic acid). A 5-ml aliquot of the sample (~1.5mM) was injected onto C18 PepMap 300 column (300mm i.d. × 15 cm, 300 Å, Thermo F ...

fluorochrome-antibody conjugates (all from BD Biosciences): FITC anti-mouse IgG1 (A85-1), FITC anti-mouse IgG2a/2b (R2-40), FITC ...

staining pattern of these single cells are shown in figs. S7A and S23A. B220+B cells were fur- ther gated on single cells, and t ...

The probability ofkor more events occurring inthetimeintervalisthen PðX≥kÞ¼ 1 Qðk;lÞ which can be solved forlnumerically using ...