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nature research | reporting summary

October 2018

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size

Data exclusions

No sample size calculation was performed. Group sizes of 6-10 per group were chosen EDVHGRQH[WHQVLYHLQYLYR H[SHULHQFHLQWKH

ODEto detect moderate to large (>40%) differences with ~40% standard deviations.

No data were excluded from analysis.

Replication All attempts at replication were successful3HUIRUPLQJWKHVHH[SHULPHQWVLQWKHQXPEHUVRIELRORJLFDOUHSOLFDWHVVWDWHGZLWKVWDWLVWLFDO

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Randomization Animals were randomly allocated to groups by an investigator who did not participate in any subsequent analysis.

Blinding Animals were randomly allocated to groups by an investigator who did not participate in any subsequent analysis. All analyses were

performed by blinded investigators.

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used

Validation

Antibodies were obtained from Santa Cruz (PC, PEPCK, CAMKII), Cell Signaling (ATGL, pCAMKIV, CAMKIV, pCRTC2, CRTC2, pACC,

ACC, pAMPK, AMPK, pHSL, HSL, GAPDH, and -actin), Novus Biologicals (pCAMKII), Abcam (pATGL), LSBio (pInsP3R-I), and BD

Transduction Laboratories (InsP3R-II, InsP3R-III, PKCe). The antibody to pATGL was kindly provided by Dr. Hei Sook Sul, while the

antibody to total InsP3R-I was custom-made using an epitope against the last 18 amino acids of InsP3R-I.

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Animals and other organisms

Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research

Laboratory animals Mice: male IP3R1 KO and WT controls, 10-12 weeks of age

Rats: male Sprague-Dawley rats, ~300g, 8-10 weeks of age

Wild animals The study did not involve wild animals.

Field-collected samples The study did not involve samples collected from the field.

Ethics oversight All protocols were approved by the Yale University Animal Care and Use Committee.

Note that full information on the approval of the study protocol must also be provided in the manuscript.