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nature research | reporting summary

October 2018

Field-specific reporting

Please select the one below that is the best fit for your research. If you are not sure, read the appropriate sections before making your selection.

Life sciences Behavioural & social sciences Ecological, evolutionary & environmental sciences

For a reference copy of the document with all sections, see nature.com/documents/nr-reporting-summary-flat.pdf

Life sciences study design

All studies must disclose on these points even when the disclosure is negative.

Sample size No statistical methods were used to predetermine sample sizes. Sample sizes were selected empirically from previous experimental

experience with similar assays, and/or from sizes generally employed in the field.

Data exclusions No exclusion of data was made.

Replication Experiments were performed at least 3 times and/or with sufficient cells/animals per group to demonstrate statistical significance. All

attempts at replication were successful.

Randomization This is applicable to the in vivo animal experiments: Mice were divided randomly into cages before tumor resection and treated with

corresponding drugs postresection.

Blinding The performer of the animal experiment was blinded to the randomization process. Pathological examination was done in a blinded fashion.

Reporting for specific materials, systems and methods

We require information from authors about some types of materials, experimental systems and methods used in many studies. Here, indicate whether each material,

system or method listed is relevant to your study. If you are not sure if a list item applies to your research, read the appropriate section before selecting a response.

Materials & experimental systems

n/a Involved in the study

Antibodies

Eukaryotic cell lines

Palaeontology

Animals and other organisms

Human research participants

Clinical data

Methods

n/a Involved in the study

ChIP-seq

Flow cytometry

MRI-based neuroimaging

Antibodies

Antibodies used Antibodies were used for flow cytometry, immunoblotting, immunofluorescence and ELISA.

• Flow cytometry was conducted using fluorochrome-conjugated antibodies.


• For staining of mouse cells we used: anti-CD45(Biolegend, 103116),anti-CD11b(BD biosciences, 563168),anti-Gr-1(BD
  biosciences, 553127),anti-CD3(BD biosciences, 552774),anti-CD4(BD biosciences, 562891),anti-CD8a(Biolegend, 100711),anti-
  CD25(BD biosciences, 552880),anti-Foxp3(BD biosciences, 560414),anti-CD45.1(Biolegend, 110708),anti-CD192(Biolegend,
  150610),anti-Ly6C(Biolegend, 128008),anti-Ly6G(Biolegend, 127606),anti-I-A/I-E(BD biosciences, 562564),anti-CD11c(BD
  biosciences, 550261),anti-CD24(BD biosciences, 564237),anti-CD64(BD biosciences, 740622),anti-CD16/32(Biolegend,
  101320),anti-F4/80(Biolegend, 123145),anti-CXCR1(BD biosciences, 566383),anti-CXCR2(Biolegend, 149609),anti-
  Ki67(Biolegend, 652409). All antibodies were used at 1:100 dilution for flow cytometry staining.
  -Immunoblot: anti-MMP9(abcam, ab38898),anti-TGF-beta(abcam, ab179695),anti-ARG1(abcam, ab91279),anti-VEGF-A(abcam,
  ab214424),anti-S100A8(abcam, ab92331),anti-TNF-alpha(abcam, ab183218),anti-IL-6(Cell Signaling Technology, 12912),anti-
  EGR1(Abcam, ab133695),anti-EGR2(Abcam, ab108399),anti-PPAR-gamma(Cell Signaling Technology, 2435),anti-Maf-b(Santa
  Cruz Biotechnology, sc-376387),anti-p50(Cell Signaling Technology, 55764),anti-p52(Cell Signaling Technology, 4882),anti-Rel-
  B(Cell Signaling Technology, 55764),anti-p65(Cell Signaling Technology, 55764),anti-beta-actin(Sigma, A5441),anti-DNMT1(Cell
  Signaling Technology, 5032),and anti-rabbit IgG, HRP-linked antibody and anti-mouse IgG, HRP-linked antibody(Cell Signaling
  Technology, 7074, 7076). All primary antibodies were used at 1:1000 dilution for immunoblotting, except anti-Maf-b was used at
  1:100 and anti-beta-actin was used at 1:10000. Secondary antibodies were used at a 1:2000 dilution.


• Immunofluorescence analysis: Anti-Gr-1 antibody (Biolegend, 108401, 1:500),anti-GFP antibody(Abcam, ab183734, 1:200),
  anti-CD4 antibody (Abcam, ab183685, 1:200), anti-CD8a antibody (ebioscience, 14-0808-80, 1:100),and goat anti-rabbit and anti-
  rat secondary antibodies(Invitrogen, A27034, A18876).


• IFN-gamma ELISA kits: Mouse IFN-gamma (DY485) from R&D.


• For in vivo CD4/CD8 depletion we used: anti-mouse CD4 antibody(BE0003-1) , anti-mouse CD8a antibody(BE0118) and isotype
  purchased from BioXcell.