Article
Extended Data Fig. 1 | Myeloid proliferative disease with MDS features in
DNA-PKc sKD/KDTp53−/− mice. a, Representative f luorescence-activated cell
sorting (FACS) analyses of pro-B cell lymphomas from DNA-PKcsKD/KDTp 5 3−/−
mice. b, The frequency of megakaryocytes with hyperchromatic or pyknotic
nuclei and total megakaryocytes per 400× field in the spleen. Eight fields from
three independent DNA-PKcsKD/KDTp 5 3−/− mice were quantified and are
presented here. Mean ± s.e.m.; two-sided unpaired student’s t-test, **P < 0.01,
***P < 0.001. c, Representative FACS analyses of myeloid proliferative disease
in DNA-PKcsKD/KDTp 5 3−/− mice. The CD19-negative bone marrow from
DNA-PKcsKD/KDTp 5 3−/− mice shows a higher percentage of myeloid cells (CD11b+)
at the expense of RBCs (TER119+). CD19+ B cells were excluded from the wild-
type bone marrow, as DNA-PKcsKD/KDTp 5 3−/− mice have no pre-B and mature B
cells (CD19+) owing to cNHEJ defects. There is also an increase in the percentage
of c-KIT+ cells among the myeloid cells (CD11b+GR1+) from DNA-PKcsKD/KDTp 5 3−/−
mice. Mid-sized reticulocytes and RBC progenitors are reduced in the
DNA-PKcsKD/KDTp 5 3−/− bone marrow. Exact P values and defined sample sizes (n)
are provided in Supplementary Data 1.