Article
Extended Data Fig. 3 | Map resolution and model validation. a, ‘Gold
standard’ FSC plots of the reconstruction of M2Rpp–βarr1–Fab30(scFv) (global
map, black) and focused refinement reconstruction for interface M2Rpp–
βarr1–Fab30(scFv) (focused map, blue). The red and brown curves represent
the correlation between model and map for the global map and the focused
map, respectively. Refined model validation was also performed by calculating
the correlation between model and focused half map 1 (green), and the
correlation between the randomly displaced model and focused half map 2
(purple). b, c, Local resolution estimation map for M2R–βarr1–Fab30(scFv) and
focused map of half-M2Rpp–βarr1–Fab30(scFv). d, Overall model fit to the
cryo-EM map. The 7TM portion (orange) comes from the full M2Rpp–βarr1–
Fab30(scFv) map contoured at σ = 7.5, whereas the βarr1–scFv portion comes
from the focused refinement of Interface M2Rpp–βarr1–Fab30(scFv)
contoured at σ = 5. 2.