nt12dreuar3esd

Extended Data Table 1 | Cryo-EM data collection, refinement, and validation statistics

M2R-arr1-

Fab30(scFv)

(EMDB-20612)

(PDB 6U1N)

InterfaceM2R-

arr1-

Fab30(scFv)

(EMDB-

20948)

Data collection and processing

Magnification 47,169 47,169

Voltage (kV) 300 300

Electron exposure (e–/Å^2 ) 50 50

Defocus range (μm) 1.2-2.2 1.2-2.2

Pixel size (Å) 1.06 1.06

Symmetry imposed C1 C1

Initial particle images (no.) 11.7million 11.7million

Final particle images (no.) 145,618 145,618

Map resolution (Å)

FSC threshold

4Å

0.143

3.6Å

0.143

Map resolution range (Å) 3.6-6 3.2-5

Refinement

Initial model used (PDB code) 6OIK

4JQI

Model resolution (Å)

FSC threshold

4.03

0.5

Map sharpening B factor (Å^2 ) 150

Model composition

Non-hydrogen atoms

Protein atoms

Ligands

5863

5834

29

B factors (Å^2 )

Protein

Ligand

164.85

292.74

R.m.s. deviations

Bond lengths (Å)

Bond angles (°)

0.005

0.682

Validation

MolProbity score

Clashscore

Poor rotamers (%)

2.2

18.0

1.0

Ramachandran plot

Favored (%)

Allowed (%)

Disallowed (%)

91.6

8.4

0.0